绵羊肝脏6-磷酸葡萄糖酸脱氢酶的化学机制。不涉及席夫碱中间体。
The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved.
作者信息
Topham C M, Dalziel K
出版信息
Biochem J. 1986 Mar 15;234(3):671-7. doi: 10.1042/bj2340671.
Abstract
[2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described.
摘要
制备了[2-¹⁸O]5-磷酸核酮糖，并证明其在绵羊肝脏的6-磷酸葡萄糖酸脱氢酶作用下酶促转化为6-磷酸葡萄糖酸，重同位素完全保留。这一发现明确排除了该酶存在席夫碱机制的可能性。金属离子的参与已经被排除，并讨论了其他可能的机制。该酶通过一种改进的大规模方法进行了纯化，在此简要描述。
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