Department of Material Science and Engineering, The Institute of Natural and Applied Sciences, Ege University, Bornova, 35100 Izmir, Turkey.
Analytical Chemistry Department, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey.
Biosensors (Basel). 2023 Apr 4;13(4):458. doi: 10.3390/bios13040458.
Selective and sensitive detection of human activated protein C (APC) was performed herein by using carbon nanofiber (CNF) and ionic liquid (IL) composite modified pencil graphite electrode (PGE) and electrochemical impedance spectroscopy (EIS) technique. A carbon nanomaterial-based electrochemical aptasensor was designed and implemented for the first time in this study for the solution-phase interaction of DNA-Apt with its cognate protein APC as well as APC inhibitor aptamer-antidote pair. The applicability of this assay developed for the determination of APC in fetal bovine serum (FBS) and its selectivity against different proteins (protein C, thrombin, bovine serum albumin) was also examined. CNF-IL modified aptasensor specific to APC provided the detection limit as 0.23 μg/mL (equal to 3.83 nM) in buffer medium and 0.11 μg/mL (equal to 1.83 nM) in FBS. The duration of the proposed assay from the point of electrode modification to the detection of APC was completed within only 55 min.
本文采用碳纳米纤维（CNF）和离子液体（IL）复合修饰的铅笔芯石墨电极（PGE）和电化学阻抗谱（EIS）技术，实现了对人激活蛋白 C（APC）的选择性和灵敏检测。本研究首次设计并实施了基于碳纳米材料的电化学适体传感器，用于 DNA-适体与靶蛋白 APC 及其 APC 抑制剂适体-解毒剂对在溶液相中的相互作用。还检查了该测定法在测定胎牛血清（FBS）中的 APC 及其对不同蛋白质（蛋白 C、凝血酶、牛血清白蛋白）的选择性的适用性。针对 APC 的 CNF-IL 修饰适体传感器在缓冲介质中的检测限为 0.23μg/mL（相当于 3.83nM），在 FBS 中的检测限为 0.11μg/mL（相当于 1.83nM）。从电极修饰到 APC 检测，该方法的整个过程仅需 55 分钟。
