School of Pharmacy, Jiangsu Key Laboratory of Inflammation and Molecular Drug Targets, Nantong University, Nantong, 226001, China; Department of Pharmacy, Affiliated Hospital of Nantong University, Nantong, 226001, China.
School of Pharmacy, Jiangsu Key Laboratory of Inflammation and Molecular Drug Targets, Nantong University, Nantong, 226001, China.
Talanta. 2023 Aug 1;260:124657. doi: 10.1016/j.talanta.2023.124657. Epub 2023 May 9.
Determining glucose in biological samples is tedious and time-consuming due to sample pretreatment. The sample is usually pretreated to remove lipids, proteins, hemocytes and other sugars that interfere with glucose detection. A surface-enhanced Raman scattering (SERS) active substrate based on hydrogel microspheres has been developed to detect glucose in biological samples. Due to the specific catalytic action of glucose oxidase (GOX), the high selectivity of detection is guaranteed. The hydrogel substrate prepared by microfluidic droplets technology protects the silver nanoparticles from the surrounding environment and improves the stability and reproducibility of the assay. In addition, the hydrogel microspheres have size-adjustable pores that selectively allow small molecules to pass through. The pores block the entry of large molecules, such as impurities, enabling glucose detection through glucose oxidase etching without sample pretreatment. This hydrogel microsphere-SERS platform is highly sensitive and enables reproducible detection of different glucose concentrations in biological samples. The use of SERS to detect glucose provides clinicians with new diagnostic methods for diabetes and a new application opportunity for SERS-based molecular detection techniques.
由于样品预处理，测定生物样品中的葡萄糖是繁琐和耗时的。通常需要对样品进行预处理以去除干扰葡萄糖检测的脂质、蛋白质、血细胞和其他糖。已经开发出基于水凝胶微球的表面增强拉曼散射（SERS）活性基底来检测生物样品中的葡萄糖。由于葡萄糖氧化酶（GOX）的特殊催化作用，保证了检测的高选择性。通过微流控液滴技术制备的水凝胶基底可以保护银纳米粒子免受周围环境的影响，提高了测定的稳定性和重现性。此外，水凝胶微球具有可调节尺寸的孔，允许小分子选择性地通过。这些孔阻止了大分子（如杂质）的进入，使得通过葡萄糖氧化酶蚀刻可以在无需样品预处理的情况下检测葡萄糖。这种水凝胶微球-SERS 平台具有高灵敏度，可以在生物样品中重现性地检测不同浓度的葡萄糖。使用 SERS 检测葡萄糖为临床医生提供了糖尿病的新诊断方法，也为基于 SERS 的分子检测技术提供了新的应用机会。
