Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
Fish Shellfish Immunol. 2023 Jul;138:108800. doi: 10.1016/j.fsi.2023.108800. Epub 2023 May 13.
Long non-coding RNAs (lncRNAs), which impact gene expression following pathogen infections, have garnered significant attention in recent years. Recent discoveries have revealed that lncRNAs play a crucial role in fish immune responses to pathogen infections. We investigated the influence of lncRNA-adm2 on the antibacterial immune response generated by Aeromonas hydrophila in grass carp (Ctenopharyngodon idella) through the adsorption of cid-miR-n3. Furthermore, we found that cid-miR-n3 interacts with lncRNA-adm2 and targets the 3' UTR of adm2. The upregulation of lncRNA-adm2 expression led to the suppression of pro-inflammatory cytokines (il-1β and il-6) in CIK cells, while anti-inflammatory cytokines (il-10) increased. Our research provides evidence that lncRNAs are involved in the antibacterial immune response of fish, expanding our understanding of the function of lncRNAs in teleosts.
长非编码 RNA（lncRNAs）在病原体感染后影响基因表达，近年来受到了广泛关注。最近的发现表明，lncRNAs 在鱼类对病原体感染的免疫反应中发挥着关键作用。我们通过吸附 cid-miR-n3 研究了 lncRNA-adm2 对草鱼（Ctenopharyngodon idella）中嗜水气单胞菌产生的抗菌免疫反应的影响。此外，我们发现 cid-miR-n3 与 lncRNA-adm2 相互作用，并靶向 adm2 的 3'UTR。lncRNA-adm2 表达的上调导致 CIK 细胞中促炎细胞因子（il-1β 和 il-6）的抑制，而抗炎细胞因子（il-10）增加。我们的研究提供了证据表明 lncRNAs 参与了鱼类的抗菌免疫反应，扩展了我们对 lncRNAs 在硬骨鱼中的功能的理解。
