The State Key Laboratory of Subtropical Silviculture, Bamboo Industry Institute, Zhejiang A&F University, HangZhou, ZheJiang, China.
PeerJ. 2023 May 9;11:e15243. doi: 10.7717/peerj.15243. eCollection 2023.
(moso bamboo) is China's most widespread bamboo species, with significant economic and ecological values. Long non-coding RNA (lncRNA) is a type of regulatory RNA that is longer than 200 nucleotides and incapable of encoding proteins, and is frequently involved in regulating biotic and abiotic stress and plant development. However, the biological functions of lncRNA in moso bamboo are unknown. In this study, a lncRNA (named ) differentially expressed following UV-B treatment was discovered in the whole transcriptome sequencing database of moso bamboo. The target genes were filtered and defined by correlation analysis of and gene expression pattern. The expression levels of and its target genes were verified using qRT-PCR. The results demonstrated that the expression levels of and its target genes increased during UV-B treatment. In transgenic seedlings and moso bamboo protoplasts, was discovered to influence the expression of its target genes when overexpressed. In addition, transgenic showed higher tolerance to UV-B stress. These results suggest that and its target genes are involved in the response of moso bamboo to UV-B stress. The novel findings would contribute to our understanding of how lncRNAs regulate the response to abiotic stresses in moso bamboo.
(毛竹)是中国分布最广的竹种,具有重要的经济和生态价值。长链非编码 RNA(lncRNA)是一种长度大于 200 个核苷酸且不能编码蛋白质的调节 RNA,常参与调节生物和非生物胁迫以及植物发育。然而,lncRNA 在毛竹中的生物学功能尚不清楚。在本研究中,我们从毛竹全转录组测序数据库中发现了一个在 UV-B 处理后差异表达的 lncRNA(命名为 )。通过 与基因表达模式的相关分析,筛选并定义了靶基因。采用 qRT-PCR 验证了 及其靶基因的表达水平。结果表明,在 UV-B 处理过程中, 和其靶基因的表达水平增加。在 转基因幼苗和毛竹原生质体中,发现过表达 时会影响其靶基因的表达。此外,转基因 表现出对 UV-B 胁迫更高的耐受性。这些结果表明 及其靶基因参与了毛竹对 UV-B 胁迫的响应。这一发现有助于我们理解 lncRNA 如何调节毛竹对非生物胁迫的响应。