Meenakshi S Iyer, Rao Madan, Mayor Satyajit, Sowdhamini Ramanathan
National Centre for Biological Sciences, TIFR, Bangalore, India.
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Front Cell Dev Biol. 2023 Apr 28;11:1168050. doi: 10.3389/fcell.2023.1168050. eCollection 2023.
Actin filaments help in maintaining the cell structure and coordinating cellular movements and cargo transport within the cell. Actin participates in the interaction with several proteins and also with itself to form the helical filamentous actin (F-actin). Actin-binding proteins (ABPs) and actin-associated proteins (AAPs) coordinate the actin filament assembly and processing, regulate the flux between globular G-actin and F-actin in the cell, and help maintain the cellular structure and integrity. We have used protein-protein interaction data available through multiple sources (STRING, BioGRID, mentha, and a few others), functional annotation, and classical actin-binding domains to identify actin-binding and actin-associated proteins in the human proteome. Here, we report 2482 AAPs and present an analysis of their structural and sequential domains, functions, evolutionary conservation, cellular localization, abundance, and tissue-specific expression patterns. This analysis provides a base for the characterization of proteins involved in actin dynamics and turnover in the cell.
肌动蛋白丝有助于维持细胞结构，并协调细胞内的运动和货物运输。肌动蛋白参与与多种蛋白质的相互作用，也与自身相互作用形成螺旋丝状肌动蛋白（F-肌动蛋白）。肌动蛋白结合蛋白（ABP）和肌动蛋白相关蛋白（AAP）协调肌动蛋白丝的组装和加工，调节细胞内球状G-肌动蛋白和F-肌动蛋白之间的通量，并有助于维持细胞结构和完整性。我们利用通过多种来源（STRING、BioGRID、mentha等）获得的蛋白质-蛋白质相互作用数据、功能注释和经典的肌动蛋白结合结构域，在人类蛋白质组中鉴定肌动蛋白结合蛋白和肌动蛋白相关蛋白。在这里，我们报告了2482种AAP，并对它们的结构和序列结构域、功能、进化保守性、细胞定位、丰度和组织特异性表达模式进行了分析。该分析为表征细胞中参与肌动蛋白动力学和周转的蛋白质提供了基础。
