Khan Asifa, Saeed Sana Tabanda, Sinha Soumya, Yadav Sujata Singh, Samad Abdul
Plant Pathology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015 India.
3 Biotech. 2023 Jun;13(6):178. doi: 10.1007/s13205-023-03606-y. Epub 2023 May 12.
Begomovirus is the largest genus of the family Geminiviridae with wide host range and responsible for a considerable amount of economic damage to many important crops globally. (Indian ginseng) is an important medicinal plant with high demand in pharmaceutical industries worldwide. During the routine survey in 2019, typical characteristic viral symptoms such as severe leaf curling, downward rolling of the leaves, vein clearing, and poor growth of Withania plants with 17-20% disease incidence were observed in Lucknow, India. Typical symptoms, abundant presence of whiteflies, PCR and RCA based detection indicated the amplification of ~ 2.7 kb and suspected the causal pathogen to be a begomovirus, associated with a betasatellite (~ 1.3 kb). Transmission electron microscopy revealed the presence of twinned particles of ~ 18-20 nm in diameter. Full genome sequencing (2758 bp) of the virus and its analysis showed only 88% sequence identity with the begomovirus sequences present in the database. Hence, based on the nomenclature guidelines we concluded that the virus associated with the present disease of is a novel begomovirus and its name is proposed as .
双生病毒是双生病毒科中最大的属，寄主范围广泛，对全球许多重要作物造成了相当大的经济损失。印度人参是一种重要的药用植物，在全球制药行业有很高的需求。在2019年的常规调查中，在印度勒克瑙观察到了印度人参植株出现典型的病毒症状，如严重的叶片卷曲、叶片向下卷曲、叶脉黄化以及生长不良，发病率为17%-20%。典型症状、大量烟粉虱的存在、基于PCR和RCA的检测表明扩增出了约2.7 kb的片段，并怀疑致病病原体是一种双生病毒，与一个β卫星（约1.3 kb）有关。透射电子显微镜显示存在直径约为18-20 nm的孪生颗粒。对该病毒进行全基因组测序（2758 bp）并分析后发现，其与数据库中存在的双生病毒序列只有88%的序列同一性。因此，根据命名准则，我们得出结论，与印度人参当前病害相关的病毒是一种新型双生病毒，并将其命名为 。
