College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.
College of Life Sciences, Neijiang Normal University, Neijiang 641100, China.
Biomolecules. 2023 Mar 25;13(4):590. doi: 10.3390/biom13040590.
The study of interaction proteins of the pathogen effector protein is an important means to analyze the disease-resistance mechanism of shoot blight. To obtain the proteins interacting with the effector ApCE22 of , 27 proteins interacting with the effector ApCE22 were initially identified via a yeast two-hybrid assay, of which four interaction proteins were obtained after one-to-one validation. The B2 protein and the chaperone protein DnaJ chloroplast protein were then verified to interact with the ApCE22 effector protein by bimolecular fluorescence complementation and GST pull-down methods. Advanced structure prediction showed that the B2 protein contained the DCD functional domain related to plant development and cell death, and the DnaJ protein contained the DnaJ domain related to stress resistance. The results showed that both the B2 protein and DnaJ protein in were the target interaction proteins of the ApCE22 effector of and related to the stress resistance of the host . The successful identification of the pathogen effector interaction target protein in plays an important role in the mechanism of pathogen-host interaction, thus providing a theoretical basis for the control of shoot blight.
研究病原菌效应蛋白的互作蛋白是分析茎枯病抗性机制的重要手段。为了获得与病原菌效应蛋白 ApCE22 互作的蛋白，本研究通过酵母双杂交实验初步鉴定了 27 个与效应蛋白 ApCE22 互作的蛋白，经过一对一验证后获得了 4 个互作蛋白。然后通过双分子荧光互补和 GST 下拉实验验证了 B2 蛋白和伴侣蛋白 DnaJ 叶绿体蛋白与 ApCE22 效应蛋白互作。高级结构预测表明，B2 蛋白含有与植物发育和细胞死亡相关的 DCD 功能域，而 DnaJ 蛋白含有与应激抗性相关的 DnaJ 结构域。结果表明，均在 中是 ApCE22 效应蛋白的靶标互作蛋白，与宿主的应激抗性有关。成功鉴定病原菌效应蛋白在 中的互作靶标蛋白，对病原菌-宿主互作机制的研究具有重要意义，为茎枯病的防治提供了理论依据。
