College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.
National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, Chengdu 611130, China.
Int J Mol Sci. 2022 Jun 17;23(12):6760. doi: 10.3390/ijms23126760.
shoot blight caused by is a fungal disease that has affected a large area in China in recent years. However, it is not clear which genes are responsible for the disease resistance of × . Based on the analysis of transcriptome and proteome data, two genes, and , which may be involved in disease resistance, were screened. Two gene expression-interfering varieties, COF RNAi and CAD RNAi were successfully obtained using RNAi technology. Quantitative real-time fluorescence (qRT-PCR) results showed that gene, gene and seven related genes expression was down-regulated in the transformed varieties. After inoculating pathogen spore suspension, the incidence and disease index of cof-RNAi and cad-RNAi transformed plants increased significantly. At the same time, it was found that the content of total lignin and flavonoids in the two transformed varieties were significantly lower than that of the wild-type. The subcellular localization results showed that both CCoAOMT2 and CAD5 were localized in the nucleus and cytoplasm. The above results confirm that the and genes are involved in the resistance of to shoot blight through regulating the synthesis of lignin and flavonoids.
由 引起的疫病是近年来在中国大面积发生的一种真菌病害。然而，目前尚不清楚 × 抗疫病的抗性基因是哪些。基于转录组和蛋白质组数据分析，筛选到两个可能参与抗病的基因 和 。利用 RNAi 技术成功获得了两个基因表达干扰品种 COF RNAi 和 CAD RNAi。定量实时荧光（qRT-PCR）结果表明，转染品种中 基因、 基因和 7 个相关基因的表达均下调。接种病原菌孢子悬浮液后，cof-RNAi 和 cad-RNAi 转化植株的发病率和病情指数显著增加。同时发现，两个转化品种的总木质素和类黄酮含量明显低于野生型。亚细胞定位结果表明，CCoAOMT2 和 CAD5 均定位于细胞核和细胞质。上述结果证实， 和 基因通过调节木质素和类黄酮的合成参与了 对疫病的抗性。
