Jiang Yuan-Hong, Jhang Jia-Fong, Wu Ya-Hui, Kuo Hann-Chorng
Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.
Department of Urology, School of Medicine, Tzu Chi University, Hualien 970, Taiwan.
Biomedicines. 2023 Apr 17;11(4):1191. doi: 10.3390/biomedicines11041191.
Bladder inflammation and tissue hypoxia were considered important pathognomonic bladder features in detrusor underactivity (DU) and detrusor overactivity (DO) patients. This study investigated urine inflammatory and oxidative stress biomarker levels in DU and DO with DU (DO-DU) patients. Urine samples were collected from 50 DU and 18 DO-DU patients, as well as 20 controls. The targeted analytes included three oxidative stress biomarkers (8-OHdG, 8-isoprostane, and total antioxidant capacity [TAC]) and 33 cytokines. DU and DO-DU patients had different urine biomarker profiles from controls, including 8-OHdG, PGE2, EGF, TNFα, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-17A, and CXCL10. Controlling for age and sex, multivariate logistic-regression models revealed that 8-OHdG, PGE2, EGF, IL-5, IL-8, IL-10, and TAC were significant biomarkers for diagnosing DU. In DU patients, urine TAC and PGE levels were positively correlated with detrusor voiding pressure. In DO-DU patients, urine 8-OHdG, PGE, IL-6, IL-10, and MIP-1α levels were positively correlated with maximal urinary flow rate, while urine IL-5, IL-10, and MIP-1α were negatively correlated with the first sensation of bladder filling. Urine inflammatory and oxidative stress biomarker analysis provides a non-invasive and convenient approach for important clinical information in DU and DO-DU patients.
膀胱炎症和组织缺氧被认为是逼尿肌活动低下（DU）和逼尿肌活动亢进（DO）患者重要的膀胱特征性表现。本研究调查了DU患者以及合并DU的DO（DO-DU）患者尿液中的炎症和氧化应激生物标志物水平。收集了50例DU患者、18例DO-DU患者以及20例对照者的尿液样本。目标分析物包括三种氧化应激生物标志物（8-羟基脱氧鸟苷、8-异前列腺素和总抗氧化能力[TAC]）以及33种细胞因子。DU患者和DO-DU患者的尿液生物标志物谱与对照者不同，包括8-羟基脱氧鸟苷、前列腺素E2、表皮生长因子、肿瘤坏死因子α、白细胞介素-1β、白细胞介素-5、白细胞介素-6、白细胞介素-8、白细胞介素-10、白细胞介素-17A和CXC趋化因子配体10。在控制年龄和性别后，多因素逻辑回归模型显示，8-羟基脱氧鸟苷、前列腺素E2、表皮生长因子、白细胞介素-5、白细胞介素-8、白细胞介素-10和TAC是诊断DU的重要生物标志物。在DU患者中，尿液TAC和前列腺素E水平与逼尿肌排尿压力呈正相关。在DO-DU患者中，尿液8-羟基脱氧鸟苷、前列腺素E、白细胞介素-6、白细胞介素-10和巨噬细胞炎性蛋白-1α水平与最大尿流率呈正相关，而尿液白细胞介素-5、白细胞介素-10和巨噬细胞炎性蛋白-1α与膀胱充盈初感呈负相关。尿液炎症和氧化应激生物标志物分析为DU和DO-DU患者提供重要临床信息提供了一种非侵入性且便捷的方法。
