Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Department of Urology, School of Medicine, Tzu Chi University, Hualien, Taiwan.
Sci Rep. 2022 Apr 22;12(1):6608. doi: 10.1038/s41598-022-10696-w.
The current study aimed to investigate the diagnostic and prognostic value of urine biomarkers among female patients with dysfunctional voiding (DV). Urine samples were collected from 43 female patients with DV and 25 controls. Oxidative stress biomarkers (8-hydroxy-2-deoxyguanosine [8-OHdG], 8-isoprostane, and total antioxidant capacity [TAC]) and inflammatory markers (interleukin-1 beta [IL-1β], IL-2, IL-6, IL-8, tumor necrosis factor alpha, nerve growth factor, and brain-derived neurotrophic factor) levels were analyzed. In total, 26 patients with DV received further treatment with biofeedback pelvic floor muscle exercise or external urethral sphincter botulinum toxin A injections. Patients with DV had significantly higher urine 8-OHdG, IL-1β, IL-8, and brain-derived neurotrophic factor levels than controls. Both urine 8-OHdG and IL-1β levels were positively correlated with clinical symptoms. Patients with DV who had successful treatment outcomes had significantly lower pretreatment urine 8-isoprostane and TAC levels than those with unsuccessful outcomes. The pretreatment urine TAC level was the only independent predictor of successful treatment outcomes (odds ratio: 0.995). Compared with controls, female patients with DV had distinct urine oxidative stress biomarker and inflammatory marker profiles, which also mapped their clinical characteristics and treatment outcomes. These urine analytes might have diagnostic and prognostic values among female patients with DV.
本研究旨在探讨女性排尿功能障碍（DV）患者尿液生物标志物的诊断和预后价值。收集了 43 例女性 DV 患者和 25 例对照者的尿液样本。分析了氧化应激生物标志物（8-羟基-2-脱氧鸟苷[8-OHdG]、8-异前列腺素和总抗氧化能力[TAC]）和炎症标志物（白细胞介素-1β[IL-1β]、IL-2、IL-6、IL-8、肿瘤坏死因子-α、神经生长因子和脑源性神经营养因子）水平。共有 26 例 DV 患者接受生物反馈盆底肌锻炼或外部尿道括约肌肉毒毒素 A 注射治疗。DV 患者的尿液 8-OHdG、IL-1β、IL-8 和脑源性神经营养因子水平明显高于对照组。尿液 8-OHdG 和 IL-1β 水平与临床症状呈正相关。治疗效果良好的 DV 患者的尿液 8-异前列腺素和 TAC 水平在治疗前明显低于治疗效果不佳的患者。尿液 TAC 水平是治疗效果良好的唯一独立预测因子（比值比：0.995）。与对照组相比，女性 DV 患者尿液氧化应激生物标志物和炎症标志物谱明显不同，也反映了其临床特征和治疗效果。这些尿液分析物可能在女性 DV 患者中有诊断和预后价值。
