Yechiel E, Barenholz Y
Biochim Biophys Acta. 1986 Jul 10;859(1):105-9. doi: 10.1016/0005-2736(86)90323-8.
Cultured heart cells serve as a common model for studying the electronphysiology and pharmacology of intact cells of the myocardium from which they are derived (Sperelakis, N. (1982) in Cardiovascular Toxicology (Van Stel, E.W., ed.), pp. 57-108, Raven Press, New York). In this study, heart cell reaggregates were used for investigating the relationship between lipid composition and aging of the heart cells. Spherical reaggregates were prepared from newborn, 3- and 18-month-old rats, respectively. They were grown for 6 days in culture and then analyzed for their lipid composition and creatine phosphokinase levels. There was an age-related increase in total phospholipids and cholesterol level per unit of cell protein. Due to a relatively greater increase in the cholesterol, the mole ratio of cholesterol to phospholipids increased with animal age. The phospholipid composition was also affected. Thus, sphingomyelin levels increased, while those of phosphatidylcholine decreased; these alterations became much more pronounced with increasing animal age. All these changes could be affected by adding small unilamellar vesicles composed of egg phosphatidylcholine to the growth medium on the 5th day after seeding. Such treatment resulted in a lesser ratio of cholesterol to phospholipid as well as sphingomyelin to phosphatidylcholine, without reducing the total phospholipid per unit protein; the level of creatine phosphokinase was also reduced. This study demonstrated that cultured heart reaggregates can serve as a model for studying aging of the whole animal. Its main advantage is the ability to employ cells from rats of any desired age. Currently this is not possible for cultured heart monolayers.
培养的心脏细胞是研究其来源的完整心肌细胞电生理学和药理学的常用模型（斯佩雷拉基斯，N.（1982年），载于《心血管毒理学》（范·斯泰尔，E.W.编），第57 - 108页，拉文出版社，纽约）。在本研究中，心脏细胞团聚体被用于研究脂质组成与心脏细胞衰老之间的关系。分别从新生、3个月和18个月大的大鼠制备球形团聚体。它们在培养中生长6天，然后分析其脂质组成和肌酸磷酸激酶水平。单位细胞蛋白中的总磷脂和胆固醇水平随年龄增长而增加。由于胆固醇增加相对较多，胆固醇与磷脂的摩尔比随动物年龄增加。磷脂组成也受到影响。因此，鞘磷脂水平增加，而磷脂酰胆碱水平下降；随着动物年龄增加，这些变化变得更加明显。在接种后第5天向生长培养基中添加由卵磷脂酰胆碱组成的小单层囊泡，所有这些变化可能会受到影响。这种处理导致胆固醇与磷脂以及鞘磷脂与磷脂酰胆碱的比例降低，而不降低单位蛋白中的总磷脂；肌酸磷酸激酶水平也降低。这项研究表明，培养的心脏团聚体可作为研究整个动物衰老的模型。其主要优点是能够使用任何所需年龄大鼠的细胞。目前，对于培养的心脏单层细胞来说这是不可能的。
