Strain dependence of the metabolism of cis- and trans-isomers of 9-octadecenoic acid in perfused liver and cell-free preparation in rats.

Hepatic metabolism of cis- and trans-9-octadecenoic acid was compared in various strains of rats and under different nutritional states. In Wistar rats triacylglycerol secretion was consistently higher in livers perfused with the cis isomer than with the trans isomer, while the difference was considerably attenuated in Sprague-Dawley rats. The difference in the hepatic triacylglycerol secretion disappeared when rats were fasted for 2 days. The rate of oxidation of trans fatty acid to ketone bodies was remarkably much higher than the cis isomer in Wistar but not in Sprague-Dawley rats. After fasting, the difference in the ketone body production disappeared in Wistar rats, whereas the oxidation rate was rather lower in the trans isomer than in the cis isomer in Sprague-Dawley rats. In isolated mitochondria, ketogenesis from trans-9-octadecenoic acid was markedly lower than that from the cis counterpart, irrespective of the nutritional states or strains of rats, and correlated well with the substrate specificity of carnitine acyltransferase. The molar concentration of malonyl-CoA to cause 50% inhibition of ketogenesis, the rate of peroxisomal beta-oxidation and the activity of acyl-CoA oxidase were all comparable, irrespective of the substrate sources. The Km value for acyl-CoA oxidase to the trans-acyl-CoA was 2-times higher than that of the cis counterpart in both strains of rats. Thus, peroxisomal as well as mitochondrial fatty acid oxidation systems apparently discriminated between the geometrical differences of the fatty acid substrate.