1st Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Alexandra Hospital, National and Kapodistrian University of Athens, 11528 Athens, Greece.
Department of Biological Chemistry, Medical School, National and Kapodistrial University of Athens, 11528 Athens, Greece.
Cells. 2023 Apr 18;12(8):1183. doi: 10.3390/cells12081183.
The presence of stem cells has been previously described in human precancerous and malignant cervical cultures. Previous studies have shown a direct interplay of the stem cell niche, which is present in practically every tissue with the extracellular matrix. In the present study, we sought to determine the expression of stemness markers in cytological specimens collected from the ectocervix among women with cervical insufficiency during the second trimester of pregnancy and women with normal cervical length. A prospective cohort of 59 women was enrolled of whom 41 were diagnosed with cervical insufficiency. The expression of OCT-4 and NANOG was higher in the cervical insufficiency group compared to the control group (-5.03 (-6.27, -3.72) vs. -5.81 (-7.67, -5.02) = 0.040 for OCT4) and (-7.47 (-8.78, -6.27) vs. -8.5 (-10.75, -7.14), = 0.035 for NANOG. Differences in the DAZL gene were not significantly different (5.94 (4.82, 7.14) vs. 6.98 (5.87, 7.43) = 0.097). Pearson correlation analysis indicated the existence of a moderate correlation of OCT-4 and Nanog with cervical length. Considering this information, the enhanced activity of stemness biomarkers among pregnant women diagnosed with cervical insufficiency may be predisposed to cervical insufficiency, and its predictive accuracy remains to be noted in larger population sizes.
先前已经在人癌前和恶性宫颈培养物中描述了干细胞的存在。先前的研究表明，干细胞生态位与细胞外基质之间存在直接相互作用，而这种干细胞生态位几乎存在于所有具有细胞外基质的组织中。在本研究中，我们试图确定在妊娠中期患有宫颈机能不全的女性和宫颈长度正常的女性的宫颈外口细胞学标本中干细胞标志物的表达。一项前瞻性队列研究纳入了 59 名女性，其中 41 名被诊断为宫颈机能不全。与对照组相比，宫颈机能不全组 OCT-4 和 NANOG 的表达更高（-5.03（-6.27，-3.72）与-5.81（-7.67，-5.02），OCT4 = 0.040）和（-7.47（-8.78，-6.27）与-8.5（-10.75，-7.14），NANOG = 0.035）。DAZL 基因的差异无显著差异（5.94（4.82，7.14）与 6.98（5.87，7.43），= 0.097）。Pearson 相关分析表明，OCT-4 和 Nanog 与宫颈长度之间存在中度相关性。考虑到这些信息，诊断为宫颈机能不全的孕妇中干细胞标志物的活性增强可能容易发生宫颈机能不全，但其预测准确性仍需在更大的人群中注意。
