多重单细胞转录组分析揭示了猴多能干细胞系的分子特征。
Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines.
机构信息
State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.
Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China.
出版信息
J Zhejiang Univ Sci B. 2023 May 15;24(5):418-429. doi: 10.1631/jzus.B2200440.
Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to monkey cells and provides guidance for exploring chemical cocktails for monkey stem cell line derivation.
已经做出了各种努力来建立人类多能干细胞系。然而，这种方法尚未在非人类灵长类动物细胞中复制。在这里，我们介绍了一种多重单细胞测序技术，用于分析已发表培养条件下灵长类多能干细胞的分子特征。结果表明，多能性的维持并不理想，并且表明用于重置人类干细胞的选定信号通路也可以用于建立猴细胞系。总的来说，这项工作使将新型人类细胞系培养条件转化为猴细胞合法化，并为探索猴干细胞系衍生的化学鸡尾酒提供了指导。
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