State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Yunnan, Kunming, China.
State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Yunnan, Kunming, China.
Trends Biotechnol. 2021 Dec;39(12):1332-1342. doi: 10.1016/j.tibtech.2021.03.010. Epub 2021 Apr 30.
The improved ability to organize pluripotent stem cells (PSCs) into 3D structures in vitro has shed light on organoid technology to recapitulate organs and tumors in vivo. Advances in gene-editing technologies, particularly CRISPR-mediated techniques, offer tremendous potential in facilitating organoid research, including the study of development, disease modeling, and personalized medicine. This review discusses how the combination of two novel technologies - organoids and gene editing - not only contributes to revealing molecular events taking place during development and tumorigenesis but also has implications for biobanking, precision medicine, and other diverse biomedical applications.
体外将多能干细胞（PSCs）组织成 3D 结构的能力的提高，为类器官技术在体内重现器官和肿瘤提供了思路。基因编辑技术的进步，特别是 CRISPR 介导的技术，为类器官研究提供了巨大的潜力，包括发育、疾病建模和个性化医学的研究。本文讨论了这两种新技术——类器官和基因编辑的结合，不仅有助于揭示发育和肿瘤发生过程中发生的分子事件，而且对生物库、精准医学和其他多样化的生物医学应用也具有重要意义。
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