Krause P J, Malech H L, Kristie J, Kosciol C M, Herson V C, Eisenfeld L, Pastuszak W T, Kraus A, Seligmann B
Blood. 1986 Jul;68(1):200-4.
We have used a mouse monoclonal antibody (31D8) to determine whether differences in neutrophil (PMN) subpopulations might help explain decreased PMN chemotaxis in neonates compared with that of adults. 31D8 has been shown to bind heterogeneously to adult PMNs. Approximately 80% of the PMNs that strongly bind 31D8 (31D8 "bright") are the same cells that depolarize and migrate chemotactically when stimulated with the chemoattractant N-formyl-methionylleucylphenylalanine, while the 20% that weakly bind 31D8 fail to similarly respond. All neonatal PMNs bound 31D8 heterogeneously. There was a smaller population of 31D8 "bright" cells in neonates at birth (76% +/- 6%, n = 45) compared with that of neonates at three to 15 days of age (82% +/- 5%, n = 10, P less than 0.002) and both were smaller than that of adults (88% +/- 4%, n = 45, P less than 0.001 and P less than 0.001). Neonatal cord PMNs, which traversed a micropore filter in a modified Boyden chemotaxis chamber in the presence of a chemoattractant, had an increased percentage of 31D8 "bright" cells (89% +/- 7%) than did PMNs which remained above the filter (82% +/- 7%, n = 10, P = 0.034). PMN chemotaxis was less in neonates at birth (32.7 +/- 4.5 micron) than at three to six days of age (36.8 +/- 11.3 micron) and both were decreased compared with that of adults (69.1 +/- 12.4 micron, P less than 0.001 and P less than 0.001). These findings indicate that decreased PMN chemotaxis in neonates may be due in part to a smaller PMN subpopulation of highly motile cells.
我们使用了一种小鼠单克隆抗体（31D8）来确定中性粒细胞（PMN）亚群的差异是否有助于解释与成年人相比新生儿PMN趋化性降低的原因。已证明31D8与成人PMN的结合存在异质性。约80%强烈结合31D8的PMN（31D8“明亮”细胞）与在用趋化因子N-甲酰甲硫氨酰亮氨酰苯丙氨酸刺激时发生去极化并进行趋化迁移的细胞相同，而20%弱结合31D8的细胞则没有类似反应。所有新生儿PMN与31D8的结合均存在异质性。出生时新生儿的31D8“明亮”细胞群体（76%±6%，n = 45）比3至15日龄的新生儿（82%±5%，n = 10，P<0.002）小，且两者均小于成年人（88%±4%，n = 45，P<0.001和P<0.001）。在趋化因子存在的情况下，在改良的Boyden趋化性小室中穿过微孔滤膜的新生儿脐带PMN中，31D8“明亮”细胞的百分比（89%±7%）高于留在滤膜上方的PMN（82%±7%，n = 10，P = 0.034）。出生时新生儿的PMN趋化性（32.7±4.5微米）低于3至6日龄时（36.8±11.3微米），且两者均低于成年人（69.1±12.4微米，P<0.001和P<0.001）。这些发现表明，新生儿PMN趋化性降低可能部分归因于高迁移性细胞的PMN亚群较小。
