de Mello Pedrosa Simone Monte Bandeira, de Lima Pontes Venâncio Anna Kelly, Lopes Levi Auto, da Silva Tomás Guilherme Pereira, de Carvalho Francisco Fernando Ramos, da Silva Júnior Valdemiro Amaro
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Dom Manuel de Medeiros Street, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
Department of Animal Science, Federal Rural University of Pernambuco, Dom Manuel de Medeiros Street, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
Trop Anim Health Prod. 2023 May 16;55(3):202. doi: 10.1007/s11250-023-03618-z.
The aim of this study was to evaluate the effects of diets containing cactus cladodes genotypes on plasma testosterone levels, testicular histopathological and histomorphometric parameters, and oxidative stress markers in lambs. Thirty-six male, intact Santa Inês lambs (22.0 ± 2.9 kg initial body weight), were to feedlot for 86 days. A completely randomized design was used with three dietary treatments (control diet with Tifton-85 hay as the only roughage; and two more diets with Miúda or OEM cactus cladodes partially replacing hay) and twelve replicates. There was no influence of the diets on the testicular weight (P = 0.414) and gonadosomatic index (P = 0.384) of lambs. The testosterone serum concentrations were almost twice as higher in lambs fed Miúda cactus cladodes compared to control treatment. There was greater incidence and severity of lesions in the testicular parenchyma of animals that received control diet: loosening of germ cell epithelium, germ cell desquamation and vacuolization of Sertoli cells. The seminiferous tubule diameter and height of the seminiferous epithelium were higher in lambs fed OEM cactus cladodes (P = 0.003). The tubular volume and Leydig cells volume were higher in animals fed with cactus cladodes (P < 0.05). The levels of malondialdehyde were higher in the lambs of control group compared to OEM group (P = 0.039) and the testicular concentration of nitric oxide was higher in control group (P = 0.009). The diet containing OEM cactus cladodes increased the levels of superoxide dismutase. Our results indicate that diets containing cactus cladodes promote antioxidant protection to the testicular parenchyma and preserve the spermatogenic process of lambs.
本研究旨在评估含有不同仙人掌茎片基因型的日粮对羔羊血浆睾酮水平、睾丸组织病理学和组织形态计量学参数以及氧化应激标志物的影响。三十六只雄性未阉割的圣伊内斯羔羊（初始体重22.0±2.9千克），在育肥场饲养86天。采用完全随机设计，有三种日粮处理（以蒂夫顿85干草作为唯一粗饲料的对照日粮；另外两种日粮用米乌达或OEM仙人掌茎片部分替代干草），每组十二个重复。日粮对羔羊的睾丸重量（P = 0.414）和性腺指数（P = 0.384）没有影响。与对照处理相比，饲喂米乌达仙人掌茎片的羔羊血清睾酮浓度几乎高出两倍。接受对照日粮的动物睾丸实质中病变的发生率和严重程度更高：生殖细胞上皮疏松、生殖细胞脱落和支持细胞空泡化。饲喂OEM仙人掌茎片的羔羊生精小管直径和生精上皮高度更高（P = 0.003）。饲喂仙人掌茎片的动物肾小管体积和睾丸间质细胞体积更高（P < 0.05）。对照组羔羊的丙二醛水平高于OEM组（P = 0.039），对照组睾丸中的一氧化氮浓度更高（P = 0.009）。含有OEM仙人掌茎片的日粮增加了超氧化物歧化酶的水平。我们的结果表明，含有仙人掌茎片的日粮对睾丸实质具有抗氧化保护作用，并能维持羔羊的生精过程。
