Silva S V, Soares A T, Batista A M, Almeida F C, Nunes J F, Peixoto C A, Guerra M M P
Andrology Laboratory, Veterinary Medicine Department, Recife, UFRPE, Brazil.
Reprod Domest Anim. 2011 Oct;46(5):874-81. doi: 10.1111/j.1439-0531.2011.01758.x. Epub 2011 Feb 21.
The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mM group had lesser TM and better STR than the other groups and the GSH 5 and 7 mM groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mM (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mM GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mM preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg-yolk extender offers protection to the membranes of sperm cells after thawing.
本研究的目的是评估在绵羊精液冷冻稀释液中添加超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）的体外和体内效果。在总活力（TM）、直线性（STR）和摆动性（WOB）方面，各实验组之间存在显著差异（p < 0.05），其中7 mM GSH组的TM低于其他组，但STR优于其他组，且GSH 5 mM和7 mM组的WOB值高于对照组、25 U/ml SOD组和100 U/ml SOD组。超微结构分析显示，100 U/ml SOD组、2 mM GSH组和5 mM GSH组冷冻后顶体的保存情况优于其他组（p < 0.05），而对照组和7 mM GSH组的线粒体损伤最大。无论实验组如何，冷冻后质膜均保持完整。在体内受精方面，SOD组的效果优于GSH组（p > 0.05）。因此可以得出结论，添加100 U/ml SOD、2 mM GSH和5 mM GSH可保持冷冻绵羊精子顶体的完整性，而在Tris蛋黄稀释液中添加100 U/ml SOD可在解冻后保护精子细胞的膜。
