Kollias G, Wrighton N, Hurst J, Grosveld F
Cell. 1986 Jul 4;46(1):89-94. doi: 10.1016/0092-8674(86)90862-7.
We have introduced the human fetal gamma- and adult beta-globin genes into the germ line of mice. Analysis of the resulting transgenic mice shows that the human gamma-globin gene is expressed like an embryonic mouse globin gene; the human beta-globin gene is expressed (as previously shown) like an adult mouse globin gene. These results imply that the regulatory signals for tissue- and developmental stage-specific expression of the globin genes have been conserved between man and mouse but that the timing of the signals has changed. Because the two genes are expressed differently, we introduced a hybrid gamma beta-globin gene construct. The combination of the regulatory sequences resulted in the expression of the hybrid gene at all stages in all the murine erythroid tissues.
我们已将人类胎儿γ-珠蛋白基因和成人β-珠蛋白基因导入小鼠种系。对所得转基因小鼠的分析表明，人类γ-珠蛋白基因的表达类似于胚胎期小鼠珠蛋白基因；人类β-珠蛋白基因的表达（如先前所示）类似于成年期小鼠珠蛋白基因。这些结果表明，珠蛋白基因在组织和发育阶段特异性表达的调控信号在人和小鼠之间是保守的，但信号的时间发生了变化。由于这两个基因的表达不同，我们构建了一个杂交γβ-珠蛋白基因。调控序列的组合导致杂交基因在所有小鼠红细胞组织的所有阶段均有表达。
