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人胚胎和滋养层干细胞的聚集揭示了滋养外胚层对内胚层分化的作用。

An aggregation of human embryonic and trophoblast stem cells reveals the role of trophectoderm on epiblast differentiation.

机构信息

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.

出版信息

Cell Prolif. 2023 May;56(5):e13492. doi: 10.1111/cpr.13492. Epub 2023 May 17.

DOI:10.1111/cpr.13492
PMID:37199067
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10212713/
Abstract

The interactions between extra-embryonic tissues and embryonic tissues are crucial to ensure proper early embryo development. However, the understanding of the crosstalk between the embryonic tissues and extra-embryonic tissues is lacking, mainly due to ethical restrictions, difficulties in obtaining natural human embryos, and lack of appropriate in vitro models. Here by aggregating human embryonic stem cells (hESCs) with human trophoblast stem cells (hTSCs), we revealed the hESCs robustly self-organized into a unique asymmetric structure which the primitive streak (PS) like cells exclusively distributed at the distal end to the TS-compartment, and morphologically flattened cells, presumed to be the extra-embryonic mesoderm cells (EXMC) like cells, were induced at the proximal end to hTSCs. Our study revealed two potential roles of extra-embryonic trophectoderm in regulating the proper PS formation during gastrulation and EXMCs induction from the human epiblast.

摘要

胚胎外组织和胚胎组织之间的相互作用对于确保早期胚胎的正常发育至关重要。然而，由于伦理限制、难以获得天然人类胚胎以及缺乏合适的体外模型，人们对胚胎组织和胚胎外组织之间的串扰了解甚少。在这里，我们通过将人类胚胎干细胞（hESCs）与人类滋养层干细胞（hTSCs）聚集在一起，揭示了 hESCs 能够强烈地自组织成一种独特的不对称结构，其中类似于原始条纹（PS）的细胞专门分布在远离 TS 隔室的远端，而在 hTSCs 的近端诱导出形态上扁平的细胞，推测是类似于胚胎外中胚层细胞（EXMC）的细胞。我们的研究揭示了胚胎外滋养外胚层在调节原条形成过程中的两个潜在作用以及从人类上胚层诱导 EXMCs。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/c4982aba1a88/CPR-56-e13492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/e6027c9197c0/CPR-56-e13492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/0124757f8d03/CPR-56-e13492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/a14263c02fb2/CPR-56-e13492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/c4982aba1a88/CPR-56-e13492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/e6027c9197c0/CPR-56-e13492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/0124757f8d03/CPR-56-e13492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/a14263c02fb2/CPR-56-e13492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68f7/10212713/c4982aba1a88/CPR-56-e13492-g001.jpg

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本文引用的文献

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Nature. 2022 Dec;612(7941):732-738. doi: 10.1038/s41586-022-05526-y. Epub 2022 Dec 14.
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Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development.由胚胎干细胞衍生而来的小鼠胚胎模型经历神经胚形成和心脏发育。
Cell Stem Cell. 2022 Oct 6;29(10):1445-1458.e8. doi: 10.1016/j.stem.2022.08.013. Epub 2022 Sep 8.
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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells.
利用原始多能干细胞对人类胚胎外中胚层细胞进行建模。
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