Wang Yiming, Wu Hao, Jiang Xiangxiang, Jia Lei, Wang Meijiao, Rong Yin, Chen Shuo, Wang Yue, Xiao Zhenyu, Liang Xiaoyan, Wang Hongmei
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Cell Dev Biol. 2022 Apr 11;10:836390. doi: 10.3389/fcell.2022.836390. eCollection 2022.
Upon implantation, the trophectoderm differentiates into the multi-nucleated primitive syncytiotrophoblast (pSTB) through a process called primary syncytialization to facilitate maternal-fetal interactions and to establish a pregnancy. However, ethical issues and limited access to human embryos around the time of embryo implantation hinder the investigation of the detailed molecular mechanisms underpinning this event in humans. Here we established human trophoblast stem cells (hTSCs) from human blastocysts. We characterized nuclear enlargement in STB differentiated from hTSCs, which recapitulate morphological nuclear features of pSTB in human embryos. Specifically, we revealed that CRISPR/Cas9-mediated disruption perturbated nuclear volume during hTSCs syncytialization. Overall, our results not only provide an interesting insight into mechanisms underlying nuclear enlargement during primary syncytialization but highlight the hTSCs as an indispensable model in understanding human trophoblast differentiation during implantation.
植入后，滋养外胚层通过称为初级合体化的过程分化为多核原始合体滋养层（pSTB），以促进母胎相互作用并建立妊娠。然而，伦理问题以及胚胎植入前后获取人类胚胎的机会有限，阻碍了对人类这一过程背后详细分子机制的研究。在此，我们从人类囊胚中建立了人类滋养层干细胞（hTSCs）。我们对从hTSCs分化而来的STB中的核增大进行了表征，这重现了人类胚胎中pSTB的形态学核特征。具体而言，我们发现CRISPR/Cas9介导的干扰在hTSCs合体化过程中扰乱了核体积。总体而言，我们的结果不仅为初级合体化过程中核增大的潜在机制提供了有趣的见解，还突出了hTSCs作为理解植入过程中人类滋养层分化不可或缺的模型。
