Gathiram P, Gaffin S L, Wells M T, Brock-Utne J G
Circ Shock. 1986;19(2):231-7.
We measured the time course of elevated plasma LPS concentration caused by a temporary intestinal ischemia using the superior mesenteric artery (SMA) occlusion shock model in anesthetized cats. The systemic plasma LPS increased from 0.075 +/- 0.006 ng/cc to 0.219 +/- 0.026 ng/cc (P less than 0.001) during the occlusion period. On release of the clamp, the plasma LPS concentration rose rapidly to 0.716 +/- 0.122 ng/cc (P less than 0.001) within 20 min. Thereafter, it declined to reach baseline levels after 100-120 min reperfusion. A total of 21 animals received IV 1.0 cc/kg antilipopolysaccharide hyperimmune equine plasma (anti-LPS) either 1.5 hr before the occlusion or at 0, 10, or 20 min after release of the occlusion. Prophylactic anti-LPS prevented any rise in plasma LPS both during and after release of the occlusion. The administration of anti-LPS during the reperfusion period completely reversed the endotoxemia caused by intestinal ischemia within 5-10 min. This rapidity of response to anti-LPS may be important in the previously reported therapeutic benefit of anti-LPS.
我们使用肠系膜上动脉（SMA）闭塞休克模型，在麻醉猫中测量了由暂时性肠缺血引起的血浆LPS浓度升高的时间进程。在闭塞期间，全身血浆LPS从0.075±0.006 ng/cc增加到0.219±0.026 ng/cc（P＜0.001）。松开夹子后，血浆LPS浓度在20分钟内迅速升至0.716±0.122 ng/cc（P＜0.001）。此后，在再灌注100 - 120分钟后降至基线水平。总共21只动物在闭塞前1.5小时或闭塞松开后0、10或20分钟接受静脉注射1.0 cc/kg抗脂多糖超免疫马血浆（抗LPS）。预防性抗LPS可防止闭塞期间和松开后血浆LPS的任何升高。在再灌注期间给予抗LPS可在5 - 10分钟内完全逆转由肠缺血引起的内毒素血症。这种对抗LPS的快速反应可能对先前报道的抗LPS治疗益处很重要。
