School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou, 510275, China.
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Adv Healthc Mater. 2023 Sep;12(24):e2300737. doi: 10.1002/adhm.202300737. Epub 2023 May 25.
Neutrophil activation is a hallmark of the immune response. Approaches to identify neutrophil activation in real time are necessary but are still lacking. In this study, magnetic Spirulina micromotors are used as label-free probes that exhibit differences in motility under different neutrophil activation states. This is correlated with different secretions into the extracellular environment by activated/non-activated cells and local environmental viscoelasticity. The micromotor platform can bypass non-activated immune cells while being stopped by activated cells. Thus, the micromotors can serve as label-free biomechanical probes of the immune cell state. They can detect the activation state of target immune cells in real time and with single-cell precision, which provides new ideas for the diagnosis and treatment of diseases while deepening understanding of the biomechanics of activated immune cells.
中性粒细胞的激活是免疫反应的一个标志。目前需要能够实时识别中性粒细胞激活的方法，但仍存在一些不足。在这项研究中，磁性螺旋藻微马达被用作无标记探针，在不同的中性粒细胞激活状态下表现出不同的运动差异。这与激活/未激活细胞向细胞外环境的不同分泌以及局部环境粘弹性有关。微马达平台可以绕过未激活的免疫细胞，而被激活的细胞所阻挡。因此，微马达可以作为免疫细胞状态的无标记生物力学探针。它们可以实时、单细胞精度地检测目标免疫细胞的激活状态，为疾病的诊断和治疗提供了新思路，同时加深了对激活免疫细胞生物力学的理解。
