National Key Laboratory of Crop Genetic Improvement, College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Institute of Nuclear and Biotechnology, Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Crop Biotechnology/The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions, Urumqi, 830091, Xinjiang, China.
Theor Appl Genet. 2023 May 18;136(6):130. doi: 10.1007/s00122-023-04379-x.
Genomic and genetic resources of G. mustelinum were effective for identifying genes for qualitative and quantitative traits. Gossypium mustelinum represents the earliest diverging evolutionary lineage of polyploid Gossypium, representing a rich gene pool for numerous desirable traits lost in cotton cultivars. Accurate information of the genomic features and the genetic architecture of objective traits are essential for the discovery and utilization of G. mustelinum genes. Here, we presented a chromosome-level genome assembly of G. mustelinum and developed an introgression population of the G. mustelinum in the background of G. hirsutum that contained 264 lines. We precisely delimited the boundaries of the 1,662 introgression segments with the help of G. mustelinum genome assembly, and 87% of crossover regions (COs) were less than 5 Kb. Genes for fuzzless and green fuzz were discovered, and a total of 14 stable QTLs were identified with 12 novel QTLs across four independent environments. A new fiber length QTL, qUHML/SFC-A11, was confined to a 177-Kb region, and GmOPB4 and GmGUAT11 were considered as the putative candidate genes as potential negative regulator for fiber length. We presented a genomic and genetic resource of G. mustelinum, which we demonstrated that it was efficient for identifying genes for qualitative and quantitative traits. Our study built a valuable foundation for cotton genetics and breeding.
棉叶野青冈的基因组和遗传资源可有效用于鉴定定性和定量性状的基因。棉叶野青冈代表了同源多倍体棉最早的进化分支，为棉花品种中丢失的许多理想性状提供了丰富的基因库。目标性状的基因组特征和遗传结构的准确信息对于棉叶野青冈基因的发现和利用至关重要。在这里，我们呈现了棉叶野青冈的染色体水平基因组组装，并在陆地棉背景中开发了一个包含 264 个系的棉叶野青冈渐渗群体。我们借助棉叶野青冈基因组组装精确划定了 1662 个渐渗片段的边界，其中 87%的交叉区域（COs）小于 5 Kb。我们发现了无绒和绿绒的基因，并在四个独立环境中总共鉴定到 14 个稳定的 QTL，其中包括 12 个新的 QTL。一个新的纤维长度 QTL，qUHML/SFC-A11，被限制在 177-Kb 区域内，GmOPB4 和 GmGUAT11 被认为是纤维长度的潜在候选基因，作为潜在的负调控因子。我们提供了棉叶野青冈的基因组和遗传资源，证明其可有效用于鉴定定性和定量性状的基因。我们的研究为棉花遗传学和育种奠定了有价值的基础。
