Nilsson O S, Bauer H C, Brosjö O, Törnkvist H
Clin Orthop Relat Res. 1986 Jun(207):239-45.
The effect of indomethacin on heterotopic and orthotopic bone formation in rats was analyzed with respect to (1) length of treatment after implantation, (2) duration of the indomethacin induced inhibition of heterotopic bone formation, and (3) influence of age of the implant recipient. Three weeks after implantation of demineralized bone matrix, the ash weight of implants from rats receiving indomethacin 2 mg/kg body weight during the entire experiment was 31% lower than that of controls. Animals treated for only six days after implantation exhibited an almost equally pronounced inhibition. However, six weeks after implantation, the inhibition caused by six days of indomethacin treatment had almost dissipated. In older rats the implants of demineralized bone matrix induces smaller volumes of new bone than in younger rats, but indomethacin causes approximately the same degree of inhibition of osteoinduction. Orthotopic bone is not affected by indomethacin treatment. This study shows that a short period of indomethacin treatment at the time of implantation of demineralized bone matrix is sufficient to reduce experimental bone formation, but the inhibitory effect slowly diminishes if the inductive process is continuous. The results indicate that the inhibition of heterotopic new bone formation by indomethacin may be mediated through reduction of the initial inflammatory response or by reduced mesenchymal cell proliferation.
（1）植入后治疗时间；（2）消炎痛诱导的异位骨形成抑制持续时间；（3）植入受体年龄的影响。脱矿骨基质植入三周后，在整个实验过程中接受2mg/kg体重消炎痛治疗的大鼠植入物的灰重比对照组低31%。植入后仅治疗六天的动物表现出几乎同样明显的抑制作用。然而，植入六周后，消炎痛治疗六天所引起的抑制作用几乎消失。在老年大鼠中，脱矿骨基质植入物诱导形成的新骨量比年轻大鼠少，但消炎痛对骨诱导的抑制程度大致相同。原位骨不受消炎痛治疗的影响。本研究表明，在脱矿骨基质植入时进行短期消炎痛治疗足以减少实验性骨形成，但如果诱导过程持续，抑制作用会缓慢减弱。结果表明，消炎痛对异位新骨形成的抑制作用可能是通过减少初始炎症反应或减少间充质细胞增殖来介导的。
