Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, CEU-Cardenal Herrera University, Tirant lo Blanc, 7, Alfara del Patriarca, 46115, Valencia, Spain.
Department of Veterinary Sciences, Veterinary Physiology Unit, Polo Universitario Annunziata, Via Palatucci 13, 98168, Messina, Italy.
Theriogenology. 2023 Aug;206:78-86. doi: 10.1016/j.theriogenology.2023.05.008. Epub 2023 May 11.
During pregnancy, maternal erythropoietic expansion and fetal development require greater mobilization of available iron (Fe) stores. These adjustments in Fe metabolism in humans and rodents are largely mediated by the hormone hepcidin (Hepc), which controls the expression of ferroportin (Fpn), a transporter responsible for exporting Fe from stores to extracellular fluid and plasma. These mechanisms based on the regulation of Hepc on the availability of Fe during gestation in healthy mares remain unknown. The objective of this study was to determine the existence of interrelationships among concentrations of Hepc, ferritin (Ferr), Fe, and estrone (E) and progesterone (P) in Spanish Purebred mares along the whole gestation. Blood samples were taken from 31 Spanish Purebred mares each month, during 11 months of pregnancy. Fe and Ferr significantly increased and Hepc decreased during pregnancy (P < 0.05). The secretion peak of estrone (E) was reached in the 5th month and progesterone (P) between the 2nd and 3rd months of gestation (P < 0.05). Fe and Ferr were weakly positively correlated (r = 0.57; P < 0.05). Fe and Ferr were negatively correlated with Hepc (r = -0.80 and r = -0.67, respectively) (P < 0.05). P was positively correlated with Hepc (r = 0.53; P < 0.05). Pregnancy in the Spanish Purebred mare was characterized by a progressive increase in Fe and Ferr and a reduction in Hepc concentrations. E was partially responsible for the suppression of Hepc; on the other hand, P induced its stimulation during pregnancy in the mare.
在妊娠期间，母体红细胞生成和胎儿发育需要更大程度地动员可用铁（Fe）储存。人类和啮齿动物的这种铁代谢调节主要是由激素hepcidin（Hepc）介导的，Hepc 控制着铁输出蛋白（Fpn）的表达，Fpn 是负责将铁从储存中输出到细胞外液和血浆的转运体。这些基于 Hepc 在健康母马妊娠期间对铁可用性的调节机制在马中仍然未知。本研究的目的是确定西班牙纯种母马整个妊娠期 Hepc、铁蛋白（Ferr）、Fe 和雌酮（E）和孕酮（P）浓度之间是否存在相互关系。在妊娠的 11 个月中，每个月从 31 匹西班牙纯种母马中采集血液样本。Fe 和 Ferr 在妊娠期间显著增加，而 Hepc 则减少（P<0.05）。雌酮（E）的分泌高峰出现在第 5 个月，孕酮（P）在妊娠的第 2 至 3 个月之间达到峰值（P<0.05）。Fe 和 Ferr 呈弱正相关（r=0.57；P<0.05）。Fe 和 Ferr 与 Hepc 呈负相关（r=-0.80 和 r=-0.67，分别）（P<0.05）。P 与 Hepc 呈正相关（r=0.53；P<0.05）。西班牙纯种母马的妊娠特征是 Fe 和 Ferr 逐渐增加，而 Hepc 浓度降低。E 部分负责抑制 Hepc；另一方面，P 在母马妊娠期间诱导其刺激。
