Department of Pathological Anatomy, Forensic Medicine and Pathological Physiology, Dnipro State Medical University, st. Vernadskoho, 9, Dnipro, Ukraine.
Department of Pathological Anatomy, Forensic Medicine and Pathological Physiology, Dnipro State Medical University, st. Vernadskoho, 9, Dnipro, Ukraine.
J Trace Elem Med Biol. 2023 Jul;78:127189. doi: 10.1016/j.jtemb.2023.127189. Epub 2023 May 5.
In connection with the widespread use of explosive devices in military conflicts, in particular in Ukraine, is relevant to detect the biometals changes in the cerebellum and determine the presence of their influence on the behavior changes of rats in the elevated plus maze in the acute period of a mild blast-traumatic brain injury (bTBI).
The selected rats were randomly divided into 3 groups: Group I - Experimental with bTBI (with an excess pressure of 26-36 kPa), Group II - Sham and Group III - Intact. Behavior studies was in the elevated plus maze. Brain spectral analysis was with using of energy dispersive X-ray fluorescence analysis, after obtaining the quantitative mass fractions of biometals, the ratios of Cu/Fe, Cu/Zn, Zn/Fe were calculated and the data between the three groups were compared.
The results showed an increase in mobility in the experimental rats, which indicates functional disorders of the cerebellum in the form of maladaptation in space. Changes in cognitive activity also is an evidence of cerebellum suppression, which is indicated by changes in vertical locomotor activity. Grooming time was shortened. We established a significant increase in Cu/Fe and Zn/Fe ratios in the cerebellum, a decrease in Cu/Zn.
Changes in the Cu/Fe, Cu/Zn, and Zn/Fe ratios in the cerebellum correlate with impaired locomotor and cognitive activity in rats in the acute posttraumatic period. Accumulation of Fe on the 1st and 3rd day leads to disturbance of the Cu and Zn balance on the 7th day and starts a "vicious cycle" of neuronal damage. Cu/Fe, Cu/Zn, and Zn/Fe imbalances are secondary factors in the pathogenesis of brain damage as a result of primary bTBI.
由于在军事冲突中广泛使用爆炸装置，特别是在乌克兰，因此需要检测小脑中生物金属的变化，并确定它们对轻度爆炸性脑损伤（bTBI）急性期间大鼠在高架十字迷宫中行为变化的影响。
选择的大鼠被随机分为 3 组：I 组-实验性 bTBI（过压 26-36 kPa）、II 组-假手术和 III 组-完整。行为研究在高架十字迷宫中进行。脑光谱分析采用能量色散 X 射线荧光分析，获得生物金属的定量质量分数后，计算 Cu/Fe、Cu/Zn 和 Zn/Fe 的比值，并比较三组之间的数据。
结果显示实验组大鼠的活动性增加，这表明小脑功能障碍以适应不良的形式出现。认知活动的变化也是小脑抑制的证据，这表现为垂直运动活动的变化。梳理时间缩短。我们发现小脑的 Cu/Fe 和 Zn/Fe 比值显著增加，Cu/Zn 比值降低。
小脑的 Cu/Fe、Cu/Zn 和 Zn/Fe 比值的变化与创伤后急性期大鼠运动和认知活动受损有关。第 1 天和第 3 天铁的积累导致第 7 天铜和锌平衡失调，并开始神经元损伤的“恶性循环”。Cu/Fe、Cu/Zn 和 Zn/Fe 失衡是原发性 bTBI 导致脑损伤发病机制中的次要因素。
