Marine College, Shandong University, Weihai 264209, China.
College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China.
Colloids Surf B Biointerfaces. 2023 Jul;227:113354. doi: 10.1016/j.colsurfb.2023.113354. Epub 2023 May 16.
The clinical treatment of infected skin injuries caused by exogenous bacteria faces great challenges. Conventional therapeutic approaches are difficult to achieve synergistic effects of infection control and induction of skin regeneration. In this study, a novel tannic acid-based physically cross-linked double network hydrogel (PDH gel) was prepared on demand by covalent cross-linking of tannic acid (TA) with polyvinyl alcohol (PVA) and chelating ligand of TA with Fe. The homogeneity of the hydrogel was achieved by the action of glycol dispersant. With the anti-inflammatory and antioxidant properties of Fe and TA, this hydrogel exhibited excellent antibacterial properties by achieving 99.69% and 99.36% bacterial inhibition against E.coli and S. aureus, respectively. Moreover, the PDH gel exhibits good biocompatibility, stretchability (up to 200%) and skin-friendliness. After 14 days of PDH-1 gel implantation in a rat model infected by S. aureus, the wound healing rate was as high as 95.21%. PDH gel-1 showed more granulation tissue, more pronounced blood vessels, higher collagen fiber density and good collagen deposition, and its recovery effect was better than that of PSH gel and PDH gel-2 in vivo. Hence, this study provides a novel avenue for the design of future clinical infected wound healing dressings.
外源性细菌引起的感染性皮肤损伤的临床治疗面临巨大挑战。传统的治疗方法很难达到抗感染和诱导皮肤再生的协同作用。在这项研究中，通过单宁酸（TA）与聚乙烯醇（PVA）的共价交联和 TA 的螯合配体与 Fe 的螯合，按需制备了一种新型的单宁酸基物理交联双网络水凝胶（PDH 水凝胶）。二醇分散剂的作用实现了水凝胶的均一性。由于 Fe 和 TA 的抗炎和抗氧化特性，该水凝胶对大肠杆菌和金黄色葡萄球菌的抑菌率分别达到 99.69%和 99.36%，表现出优异的抗菌性能。此外，PDH 水凝胶具有良好的生物相容性、拉伸性（高达 200%）和皮肤友好性。在金黄色葡萄球菌感染的大鼠模型中植入 PDH-1 水凝胶 14 天后，伤口愈合率高达 95.21%。PDH 水凝胶-1 显示出更多的肉芽组织、更明显的血管、更高的胶原纤维密度和良好的胶原沉积，其体内恢复效果优于 PSH 水凝胶和 PDH 水凝胶-2。因此，本研究为未来临床感染性伤口愈合敷料的设计提供了新途径。
