Chang Rong, Zhao Donghui, Zhang Chen, Liu Kaiyue, He Yuanmeng, Guan Fangxia, Yao Minghao
School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
School of Pharmacy, School of Biological and Food Engineering, Changzhou University, Changzhou, Jiangsu 213164, PR China.
Int J Biol Macromol. 2023 Jan 31;226:870-884. doi: 10.1016/j.ijbiomac.2022.12.116. Epub 2022 Dec 14.
Bacterial infection and subsequent reactive oxygen species (ROS) damage are major factors that delay wound healing in infected skin. Recently, photothermal therapy (PTT), as a new antibacterial method, has shown great advantages in the treatment of infected skin wound. Antibacterial and antioxidant hydrogels can reduce bacterial colonization and infection, scavenge ROS, relieve inflammation, and accelerate wound healing. In this study, an enzyme-crosslinked hyaluronic acid-tyramine (HT) hydrogel loaded with antioxidant and photothermal silver nanoparticles (AgNPs), named HTA, was developed as functional wound dressing to promote the infected skin wound healing. Natural antioxidant tannic acids (TA) were used as both reducing and stabilizing agents to facilely synthesize the silver nanoparticles capped with TA (AgNPs@TA). The incorporation of AgNPs@TA significantly enhanced the antioxidant, antibacterial, photothermal antibacterial, adhesive, and hemostatic abilities of the resulted HTA hydrogel. Besides, HTA hydrogel has rapid gelation, well injection and biocompatibility. In vivo results on the Staphylococcus aureus and Escherichia coli co-infected mouse skin wound model showed that HTA (containing 0.4 mg/mL AgNPs@TA) hydrogel combined with near infrared ray radiation highly alleviated inflammation, promoted angiogenesis, and accelerated the healing process. Therefore, this nanocomposite hydrogel wound dressing with antibacterial and antioxidant capabilities has great application potential in the treatment of infected skin wounds.
细菌感染及随后的活性氧（ROS）损伤是延迟感染皮肤伤口愈合的主要因素。近年来，光热疗法（PTT）作为一种新型抗菌方法，在感染皮肤伤口治疗中显示出巨大优势。抗菌抗氧化水凝胶可减少细菌定植与感染，清除ROS，减轻炎症，并加速伤口愈合。在本研究中，一种负载抗氧化剂和光热银纳米颗粒（AgNPs）的酶交联透明质酸 - 酪胺（HT）水凝胶，命名为HTA，被开发为功能性伤口敷料以促进感染皮肤伤口愈合。天然抗氧化剂单宁酸（TA）用作还原剂和稳定剂，以简便地合成由TA包覆的银纳米颗粒（AgNPs@TA）。AgNPs@TA的掺入显著增强了所得HTA水凝胶的抗氧化、抗菌、光热抗菌、粘附及止血能力。此外，HTA水凝胶具有快速凝胶化、良好的可注射性和生物相容性。在金黄色葡萄球菌和大肠杆菌共感染的小鼠皮肤伤口模型上的体内结果表明，HTA（含0.4mg/mL AgNPs@TA）水凝胶联合近红外辐射可高度减轻炎症、促进血管生成并加速愈合过程。因此，这种具有抗菌和抗氧化能力的纳米复合水凝胶伤口敷料在感染皮肤伤口治疗中具有巨大的应用潜力。
