Student Research Committee and Biotechnology Research Center, Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Food Chem. 2023 Oct 30;424:136346. doi: 10.1016/j.foodchem.2023.136346. Epub 2023 May 11.
The aim of this study was to synthesize of β-cyclodextrin/Vitamin D (βCD/VitD) inclusion complex and encapsulation of this complex with gelatin-coated nanoliposomes (NLPs). Fourier transform infrared spectroscopy confirmed the formation of βCD/VitD inclusion complex. In the next step, different gelatin concentrations (1, 2, and 4 mg/mL) were used to surface coat the blank NLPs. The concentration of 2 mg/mL of gelatin was chosen as the optimal concentration for coating the complex-loaded NLPs by considering particle size, morphology, and zeta potential. The particle size and zeta potential of the coated complex-loaded NLPs were 117 ± 2.55 nm and 19.8 ± 1.25 mV, respectively. The images taken by transmission electron microscopy confirmed the formation of a biopolymer layer of gelatin around the NLPs' vesicles. The complex encapsulation efficiency inside the NLPs was 81.09%. The βCD/VitD complex loaded NLPs and its coated form exhibited a controlled release profile in simulated gastrointestinal condition.
本研究旨在合成β-环糊精/维生素 D（βCD/VitD）包合物，并将其封装在明胶包被的纳米脂质体（NLPs）中。傅里叶变换红外光谱证实了βCD/VitD 包合物的形成。下一步，使用不同浓度的明胶（1、2 和 4mg/mL）对空白 NLPs 进行表面涂层。考虑到粒径、形态和 zeta 电位，选择 2mg/mL 的明胶浓度作为包载复合物的 NLPs 的最佳涂层浓度。包载复合物的 NLPs 的粒径和 zeta 电位分别为 117±2.55nm 和 19.8±1.25mV。通过透射电子显微镜拍摄的图像证实了明胶围绕 NLPs 囊泡形成了一层生物聚合物。NLPs 内复合物的包封效率为 81.09%。βCD/VitD 复合物负载的 NLPs 及其包被形式在模拟胃肠道条件下表现出了控制释放的特性。
