PlasticReconstructive, and Hand Surgery Service, Lausanne University Hospital, University of Lausanne, Lausanne, Switzerland.
Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, Lausanne, Switzerland.
J Burn Care Res. 2024 Mar 4;45(2):308-317. doi: 10.1093/jbcr/irad071.
Currently, most burn models for preclinical testing are on animals. For obvious ethical, anatomical, and physiological reasons, these models could be replaced with optimized ex vivo systems. The creation of a burn model on human skin using a pulsed dye laser could represent a relevant model for preclinical research. Six samples of excess human abdominal skin were obtained within one hour after surgery. Burn injuries were induced on small samples of cleaned skin using a pulsed dye laser on skin samples, at varying fluences, pulse numbers and illumination duration. In total, 70 burn injuries were performed on skin ex vivo before being histologically and dermato-pathologically analyzed. Irradiated burned skin samples were classified with a specified code representing burn degrees. Then, a selection of samples was inspected after 14 and 21 days to assess their capacity to heal spontaneously and re-epithelize. We determined the parameters of a pulsed dye laser inducing first, second, and third degree burns on human skin and with fixed parameters, especially superficial and deep second degree burns. After 21 days with the ex vivo model, neo-epidermis was formed. Our results showed that this simple, rapid, user-independent process creates reproducible and uniform burns of different, predictable degrees that are close to clinical reality. Human skin ex vivo models can be an alternative to and complete animal experimentation, particularly for preclinical large screening. This model could be used to foster the testing of new treatments on standardized degrees of burn injuries and thus improve therapeutic strategies.
目前，大多数用于临床前测试的烧伤模型都是基于动物的。出于明显的伦理、解剖和生理原因，这些模型可以被优化的离体系统所取代。使用脉冲染料激光在人体皮肤上创建烧伤模型，可以代表一种用于临床前研究的相关模型。在手术后一小时内从多余的人类腹部皮肤中获得了六个样本。使用脉冲染料激光在小面积的清洁皮肤样本上以不同的能量密度、脉冲数和照射持续时间诱导烧伤损伤。总共在离体皮肤上进行了 70 次烧伤损伤，然后进行组织学和皮肤病理学分析。辐照烧伤皮肤样本用代表烧伤程度的特定代码进行分类。然后，选择一些样本在 14 天和 21 天后进行检查，以评估它们自发愈合和重新上皮化的能力。我们确定了在人体皮肤上诱导一度、二度和三度烧伤的脉冲染料激光参数，并且使用固定参数可以实现特别的浅二度和深二度烧伤。在离体模型 21 天后，形成了新生表皮。我们的结果表明，这种简单、快速、用户独立的过程可以创建不同、可预测的烧伤程度，且具有可重复性和一致性，与临床实际情况接近。人体皮肤离体模型可以替代动物实验，尤其是在临床前的大规模筛选中。该模型可用于在标准化烧伤程度上测试新的治疗方法，从而改善治疗策略。
