Queensland University of Technology (QUT), Tissue Repair and Translational Physiology Program, Institute of Health and Biomedical Innovation, Brisbane, Queensland, Australia; The University of Queensland, The Christopher Chen Oocyte Biology Research Laboratory, Centre for Clinical Research, Brisbane, Queensland, Australia.
Queensland University of Technology (QUT), Faculty of Health, School of Biomedical Sciences, Brisbane, Queensland, Australia.
Burns. 2021 Mar;47(2):417-429. doi: 10.1016/j.burns.2020.07.007. Epub 2020 Jul 15.
In this study, a deep burn wound model was established using a 3D human skin equivalent (HSE) model and this was compared to native skin. HSEs were constructed from dermis derived from abdominoplasty/breast surgery and this dermal template was seeded with primary keratinocytes and fibroblasts. The HSE model was structurally similar to native skin with a stratified and differentiated epidermis. A contact burn (60 °C, 80 °C, 90 °C) was applied with a modified soldering iron and wounds were observed at day 1 and 7 after burn. The HSEs demonstrated re-growth with keratinocyte proliferation and formation of a neo-epidermis after burn injury, whereas the ex vivo native skin did not. To assess the suitability of the 3D HSE model for penetration and toxicity studies, a nanocrystalline silver dressing was applied to the model for 7 days, with and without burn injury. The effect of silver on skin re-growth and its penetration and subcellular localization was assessed in HSEs histologically and with laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). The silver treatment delayed or reduced skin re-growth, and silver particles were detected on the top of the epidermis, and within the papillary dermis. This novel in vitro 3D multicellular deep burn wound model is effective for studying the pathology and treatment of burn wound injury and is suitable for penetration and toxicity studies of wound healing treatments.
在这项研究中，使用 3D 人皮肤等效物（HSE）模型建立了深度烧伤模型，并将其与天然皮肤进行了比较。HSE 是从腹部整形/乳房手术中提取的真皮构建的，该真皮模板被原代角质形成细胞和成纤维细胞接种。HSE 模型在结构上与天然皮肤相似，具有分层和分化的表皮。使用改良的电烙铁施加接触烧伤（60°C、80°C、90°C），并在烧伤后第 1 天和第 7 天观察伤口。HSE 在烧伤损伤后表现出角质形成细胞增殖和新表皮形成的再生长，而离体天然皮肤则没有。为了评估 3D HSE 模型在渗透和毒性研究中的适用性，将纳米晶银敷料应用于模型中 7 天，有无烧伤损伤。用激光烧蚀-电感耦合等离子体质谱法（LA-ICPMS）评估银对皮肤再生长及其渗透和亚细胞定位的影响。银处理延迟或减少了皮肤再生长，并且在表皮顶部和乳头真皮内检测到银颗粒。这种新型的体外 3D 多细胞深度烧伤模型可有效研究烧伤伤口损伤的病理学和治疗方法，适用于伤口愈合治疗的渗透和毒性研究。
