Institute of Nuclear Medicine & Allied Sciences, Defence Research and Development Organization, Lucknow Road, Timarpur, Delhi 110054, India.
Institute of Nuclear Medicine & Allied Sciences, Defence Research and Development Organization, Lucknow Road, Timarpur, Delhi 110054, India.
Burns. 2020 Aug;46(5):1142-1149. doi: 10.1016/j.burns.2019.12.005. Epub 2020 Mar 10.
The currently available practices for creation of burns in the animals are mostly manual which may lead to lack of uniform wound. There is a need to develop a suitable device that could reproduce and uniformly create burn wound in animal models without the procedural variations and human variability. Present study deals with development of a burn device which has been designed for creation of animal models for burn injury.
The designed burn device comprises of two main components a heating metal stylus and the thermal sensor. Metal stylus consists of two parts with top part acts as handle and bottom part contains the aluminum probe which quickly heats and cool. The temperature monitoring sensor is attached adjacent to the tip of the probe. The temperature and timer are digitally displayed and can be adjusted as per the requirement. This device is tested for creation of uniform burn in the mice model. Animals were divided into different groups and thermal burn was generated for 60 °C, 80 °C & 100 °C respectively. Burn wound was generated dorsally on shaved skin with contact time of 40 s. Skin biopsies of burn wound were collected after 24 h for histopathology analysis to determine the burn characteristics. Blood vessels damage in the skin was detected by transillumination and digital segmentation using VesSeg tool.
The device is able to deliver the different temperature to the animal skin. After reaching the 60 °C, 80 °C & 100 °C for 40 s respectively electronic relays shut down the device. The different groups of the animals showed significant difference in burn morphology in temperature dependent manner. Non significant variation in the burn area of different experimental groups animals was observed. All three zones vis-a-vis coagulation, stasis and hyperaemia were observed at 100 °C whereas found indistinct in 80 °C and 60 °C treated groups. Histopathological studies clearly demonstrated the differences in damage induction in stratum corneum, epidermis, dermis, collagen and hair follicle at selected thermal points. Severe blood vessels damage was observed only at 100 °C. The vascular perfusion was recorded 14% and 57% higher in 60 °C and 80 °C treated animals respectively when compared to control animals. However, at 100 °C due to highest vessel damage the perfusion was reduced to 53% compared to control.
Present study demonstrates that the device is able to generate precise and uniform burn wound in mice model. The device may be useful for burn related studies and validation of burn wound care products.
目前用于在动物身上制造烧伤的方法主要是手动的，这可能导致伤口不均匀。需要开发一种合适的设备，以便在没有程序变化和人为差异的情况下，在动物模型中复制并均匀地制造烧伤伤口。本研究涉及开发一种烧伤设备，用于为烧伤损伤的动物模型的创建。
设计的烧伤设备由两个主要部件组成，一个是加热金属探针，另一个是热传感器。金属探针由两部分组成，上部分作为手柄，下部分包含快速加热和冷却的铝探针。温度监测传感器附接到探针的尖端附近。温度和计时器以数字方式显示，并可根据需要进行调整。该设备经过测试，可在小鼠模型中产生均匀的烧伤。动物被分为不同的组，分别以 60°C、80°C 和 100°C 的温度产生热烧伤。接触时间为 40 秒，在剃光的皮肤上产生背部烧伤。在 24 小时后收集烧伤伤口的皮肤活检，进行组织病理学分析以确定烧伤特征。使用 VesSeg 工具通过透射光和数字分割检测皮肤中的血管损伤。
该设备能够将不同的温度传递给动物皮肤。当分别达到 60°C、80°C 和 100°C 并持续 40 秒后，电子继电器会关闭设备。不同温度组别的动物表现出明显的依赖于温度的烧伤形态差异。不同实验组动物的烧伤面积没有明显差异。在 100°C 时观察到所有三个区域（凝固、停滞和充血），而在 80°C 和 60°C 处理组中则发现不明显。组织病理学研究清楚地表明，在选定的热点处，角质层、表皮、真皮、胶原蛋白和毛囊的损伤诱导存在差异。仅在 100°C 时观察到严重的血管损伤。与对照组相比，在 60°C 和 80°C 处理的动物中，血管灌注分别记录到 14%和 57%的增加。然而，在 100°C 时，由于血管损伤最大，灌注减少到与对照组相比的 53%。
本研究表明，该设备能够在小鼠模型中产生精确且均匀的烧伤伤口。该设备可用于烧伤相关研究和烧伤伤口护理产品的验证。
