Department of Molecular Biology, Sejong University, Seoul, South Korea.
Department of Bioindustry and Bioresource Engineering, Sejong University, Seoul, South Korea.
Plant Signal Behav. 2023 Dec 31;18(1):2213937. doi: 10.1080/15592324.2023.2213937.
Fatty acid biosynthesis 2 (FAB2) is an essential enzyme responsible for the synthesis of unsaturated fatty acids in chloroplast membrane lipids found in leaves and triacylglycerols (TAG) in seeds. FAB2 functions at the junction of saturated to unsaturated fatty acid conversion in chloroplasts by converting 18:0-ACP to 18:1-ACP. In the present study, plant growth and seed phenotypes were examined in three Arabidopsis T-DNA mutants (, , and ). The three T-DNA mutants exhibited increased 18:0 fatty acid content in both the leaves and seeds. The degree of growth inhibition of the mutant was proportional to the increase in 18:0 and decrease in 18:3 fatty acids present in the leaves. The mutation affected seed yield but not the seed phenotype. This result indicates that FAB2 affects the fatty acid composition of the leaf chloroplast membrane more than seed TAG. In summary, the characteristics of these three mutants provide information for studying leaf membrane lipid and seed oil biosynthesis.
脂肪酸生物合成 2（FAB2）是一种在叶绿体膜脂（存在于叶片中）和种子中的三酰基甘油（TAG）中合成不饱和脂肪酸的必需酶。FAB2 通过将 18:0-ACP 转化为 18:1-ACP，在叶绿体中发挥作用，在饱和脂肪酸到不饱和脂肪酸的转化中发挥作用。在本研究中，在三个拟南芥 T-DNA 突变体（fab2-1、fab2-2 和 fab2-3）中检查了植物生长和种子表型。三个 fab2 T-DNA 突变体在叶片和种子中均表现出 18:0 脂肪酸含量增加。突变体的生长抑制程度与叶片中存在的 18:0 和 18:3 脂肪酸的增加成正比。突变影响种子产量，但不影响种子表型。这一结果表明，FAB2 对叶片叶绿体膜的脂肪酸组成的影响大于种子 TAG。总之，这三个 fab2 突变体的特征为研究叶片膜脂和种子油生物合成提供了信息。
