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Theor Appl Genet. 1990 Aug;80(2):241-5. doi: 10.1007/BF00224393.
Mutants of Arabidopsis thaliana were identified by screening pedigreed M3 seed collections from EMS-treated plants for changes in fatty acid (FA) composition. The FA phenotypes of the most dramatic mutants are as follows: G30 and 1E5 (allelic) lack linolenic acid (18∶3) and are elevated in linoleic acid (18∶2); 4A5 is deficient in 18∶2 and 18∶3 and fourfold increased in oleic acid (18∶1); 9A1 lacks all FAs > C18 and is twofold increased in 18∶1; 1A9 is twofold increased in palmitic acid (16∶0) and decreased by one-half in 18∶1; 2A11 is two-to threefold increased in stearic acid (18∶0) and decreased by one-half in 18∶1. Based on segregation of F2 selfed plants derived from crosses to wild type, all of these phenotypes are the result of single gene mutations.
通过筛选 EMS 处理植株的 pedigreed M3 种子系，鉴定拟南芥突变体，以研究脂肪酸（FA）组成的变化。最显著的突变体的 FA 表型如下：G30 和 1E5（等位基因）缺乏亚麻酸（18∶3），并升高了亚油酸（18∶2）；4A5 缺乏 18∶2 和 18∶3，油酸（18∶1）增加了四倍；9A1 缺乏所有 C18 以上的 FA，18∶1 增加了两倍；1A9 棕榈酸（16∶0）增加了两倍，18∶1 减少了一半；2A11 硬脂酸（18∶0）增加了两到三倍，18∶1 减少了一半。基于与野生型杂交自交 F2 植物的分离，所有这些表型都是单基因突变的结果。
