Optical Imaging Laboratory, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, EC 2675, Miami, FL, 33174, USA.
Cardiovascular Matrix Remodeling Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA.
Ann Biomed Eng. 2023 Sep;51(9):2035-2047. doi: 10.1007/s10439-023-03229-7. Epub 2023 May 18.
Near-infrared spectroscopy (NIRS)-based peripheral perfusion, or microcirculation, can be used to assess the severity of peripheral vascular dysfunction. A low-cost, portable non-contact near-infrared optical scanner (NIROS) was developed for spatio-temporal mapping of tissue oxygenation and perfusion in tissues. In vivo validation studies were carried out on control subjects (n = 3) to assess the ability of NIROS to measure real-time oxygenation changes in response to an occlusion paradigm on the dorsum of the hand. NIROS captured real-time tissue oxygenation changes with 95% correlation when compared to a commercial device. A feasibility peripheral imaging study was performed in a mouse model (n = 5) of chronic kidney disease (CKD) induced vascular calcification to assess differences in microcirculatory peripheral tissue oxygenation. The tissue oxygenation (in terms of oxy-, deoxy-, and total hemoglobin changes) due to the occlusion paradigm was distinctly different prior to (week-6) and after the onset of vascular calcification (week-12) in the murine tails. Future work will involve extensive studies to correlate these microcirculatory tissue oxygenation changes in the peripheral tail to the vascular calcification in the heart.
近红外光谱（NIRS）的外周灌注或微循环可用于评估周围血管功能障碍的严重程度。开发了一种低成本、便携式的非接触式近红外光扫描仪（NIROS），用于组织氧合和灌注的时空映射。在对照受试者（n=3）中进行了体内验证研究，以评估 NIROS 在手背部阻塞范式下测量实时氧合变化的能力。与商业设备相比，NIROS 可捕获具有 95%相关性的实时组织氧合变化。在慢性肾脏病（CKD）诱导的血管钙化的小鼠模型中进行了可行性外周成像研究，以评估外周组织微循环氧合的差异。在血管钙化（第 12 周）之前（第 6 周）和之后（第 12 周），由于阻塞范式，鼠尾的组织氧合（以氧合、脱氧和总血红蛋白变化来表示）明显不同。未来的工作将涉及广泛的研究，将这些外周尾部的微循环组织氧合变化与心脏中的血管钙化相关联。
