pCR1质粒中EcoRI位点周围区域的限制酶切图谱及插入的卵清蛋白基因分析。

Restriction map of the region surrounding the EcoRI site in the pCR1 plasmid and analysis of an inserted ovalbumin gene.

作者信息

Wickens M P, Buell G N, Crouse G F, Carbon J, Schimke R T

出版信息

Gene. 1979 Jan;5(1):19-43. doi: 10.1016/0378-1119(79)90090-8.

PMID:372047

Abstract

We have determined a restriction map of a 1650 base pair region surrounding the EcoRI site of the bacterial plasmid, pCR1. We have used pCR1 as a vector in cloning synthetic ovalbumin double-stranded cDNA. Using the pCR1 restriction map, we have characterized the ovalbumin sequences inserted in one recombinant plasmid, pOvE12. POvE12 appears to contain all, or nearly all, of the sequences found in full length, double-stranded cDNA synthesized in vitro.

摘要

我们已经确定了细菌质粒pCR1的EcoRI位点周围一个1650碱基对区域的限制酶切图谱。我们已将pCR1用作载体来克隆合成的卵清蛋白双链cDNA。利用pCR1限制酶切图谱，我们对插入到一个重组质粒pOvE12中的卵清蛋白序列进行了表征。pOvE12似乎包含了在体外合成的全长双链cDNA中发现的所有或几乎所有序列。