体外合成的鸡卵清蛋白结构基因广泛序列的分子克隆
Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.
作者信息
Humphries P, Cochet M, Krust A, Gerlinger P, Kourilsky P, Chambon P
出版信息
Nucleic Acids Res. 1977 Jul;4(7):2389-406. doi: 10.1093/nar/4.7.2389.
Abstract
Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.
摘要
与卵清蛋白鸡信使核糖核酸互补的双链脱氧核糖核酸分子在体外合成，并使用寡聚dG-dC加尾程序整合到大肠杆菌质粒pCR1中。通过转染大肠杆菌扩增得到的杂交质粒，经杂交和凝胶电泳显示含有卵清蛋白结构基因的大量脱氧核糖核酸序列。
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