Hsu Yen-Yu, Chen Samuel J, Bernal-Chanchavac Julio, Sharma Bineet, Moghimianavval Hossein, Stephanopoulos Nicholas, Liu Allen P
Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
bioRxiv. 2023 Jun 2:2023.05.06.539684. doi: 10.1101/2023.05.06.539684.
In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.
在细胞中，膜融合由SNARE蛋白介导，其活性依赖于钙。虽然已经证明了几种非天然的膜融合机制，但很少有能对外界刺激做出反应的。在这里，我们开发了一种钙触发的DNA介导的膜融合策略，其中融合是通过表面结合的聚乙二醇链来调节的，这些聚乙二醇链可被钙激活的蛋白酶钙蛋白酶-1切割。
