利用 DNA 功能化囊泡对生物反应进行条码化。
Barcoding Biological Reactions with DNA-Functionalized Vesicles.
机构信息
Department of Chemical and Biological Engineering, Northwestern University, USA.
Department of Biomedical Engineering, Northwestern University, McCormick School of Engineering, Technological Institute, 2145 Sheridan Road, Evanston, Il, 60208, USA.
出版信息
Angew Chem Int Ed Engl. 2019 Dec 16;58(51):18683-18690. doi: 10.1002/anie.201911544. Epub 2019 Oct 31.
Targeted vesicle fusion is a promising approach to selectively control interactions between vesicle compartments and would enable the initiation of biological reactions in complex aqueous environments. Here, we explore how two features of vesicle membranes, DNA tethers and phase-segregated membranes, promote fusion between specific vesicle populations. Membrane phase-segregation provides an energetic driver for membrane fusion that increases the efficiency of DNA-mediated fusion events. The orthogonality provided by DNA tethers allows us to direct fusion and delivery of DNA cargo to specific vesicle populations. Vesicle fusion between DNA-tethered vesicles can be used to initiate in vitro protein expression to produce model soluble and membrane proteins. Engineering orthogonal fusion events between DNA-tethered vesicles provides a new strategy to control the spatiotemporal dynamics of cell-free reactions, expanding opportunities to engineer artificial cellular systems.
靶向囊泡融合是一种有前途的方法，可以选择性地控制囊泡隔室之间的相互作用，并能够在复杂的水相环境中引发生物反应。在这里，我们探讨了囊泡膜的两个特征，即 DNA 系绳和相分离的膜，如何促进特定囊泡群体之间的融合。膜相分离为膜融合提供了能量驱动，提高了 DNA 介导的融合事件的效率。DNA 系绳提供的正交性允许我们将融合和 DNA 货物递送到特定的囊泡群体。DNA 系绳囊泡之间的融合可用于启动体外蛋白质表达，以生产可溶性和膜蛋白模型。在 DNA 系绳囊泡之间构建正交融合事件为控制无细胞反应的时空动力学提供了一种新策略，扩展了设计人工细胞系统的机会。
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