Beijing Key Laboratory of Greening Plants Breeding, Beijing Academy of Forestry and Landscape Architecture, Beijing, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2023 May 19;18(5):e0285754. doi: 10.1371/journal.pone.0285754. eCollection 2023.
Investigating the genetic diversity and population structure is important in conserving narrowly distributed plants. In this study, 90 Clematis acerifolia (C. acerifolia) plants belonging to nine populations were collected from the Taihang Mountains in Beijing, Hebei, and Henan. Twenty-nine simple sequence repeats (SSR) markers developed based on RAD-seq data were used to analyze the genetic diversity and population structure of C. acerifolia. The mean PIC value for all markers was 0.2910, indicating all SSR markers showed a moderate degree of polymorphism. The expected heterozygosity of the whole populations was 0.3483, indicating the genetic diversity of both C. acerifolia var. elobata and C. acerifolia were low. The expected heterozygosity of C. acerifolia var. elobata (He = 0.2800) was higher than that of C. acerifolia (He = 0.2614). Genetic structure analysis and principal coordinate analysis demonstrated that C. acerifolia and C. acerifolia var. elobata showed great genetic differences. Molecular variance analysis (AMOVA) demonstrated that within-population genetic variation (68.31%) was the main contributor to the variation of the C. acerifolia populations. Conclusively, C. acerifolia var. elobata had higher genetic diversity than C. acerifolia, and there are significant genetic differences between C. acerifolia and C. acerifolia var. elobata, and small genetic variations within the C. acerifolia populations. Our results provide a scientific and rational basis for the conservation of C. acerifolia and provide a reference for the conservation of other cliff plants.
研究遗传多样性和种群结构对于保护分布范围狭窄的植物非常重要。本研究从北京、河北和河南的太行山地区采集了 90 株三叶木通（C. acerifolia），属于九个种群。使用基于 RAD-seq 数据开发的 29 个简单序列重复（SSR）标记来分析三叶木通的遗传多样性和种群结构。所有标记的平均 PIC 值为 0.2910，表明所有 SSR 标记均表现出中等程度的多态性。整个种群的期望杂合度为 0.3483，表明三叶木通 var. elobata 和三叶木通的遗传多样性较低。三叶木通 var. elobata 的期望杂合度（He = 0.2800）高于三叶木通（He = 0.2614）。遗传结构分析和主坐标分析表明，三叶木通和三叶木通 var. elobata 表现出很大的遗传差异。分子方差分析（AMOVA）表明，种群内遗传变异（68.31%）是三叶木通种群变异的主要原因。结论是，三叶木通 var. elobata 的遗传多样性高于三叶木通，三叶木通和三叶木通 var. elobata 之间存在显著的遗传差异，而三叶木通种群内的遗传变异较小。我们的研究结果为三叶木通的保护提供了科学合理的依据，为其他悬崖植物的保护提供了参考。
