Department of Medical Mycology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Front Cell Infect Microbiol. 2023 May 3;13:1128000. doi: 10.3389/fcimb.2023.1128000. eCollection 2023.
Previous studies have shown that natural polyacetylene alcohols, such as falcarindiol (FADOH), have good antifungal effects on plant fungi. While its effect on fungi that infect humans remains to be explored. In our study, checkerboard microdilution, drop-plate assay, and time-growth method were employed to analyze the interactions between FADOH and itraconazole (ITC) against dermatophytes, including 12 (), 12 (), and 6 (). The results showed that the combination of FADOH and ITC exhibited synergistic and additive activity against 86.7% of all tested dermatophytes. FADOH had an excellent synergistic effect on ITC against and ; the synergistic rates were 66.7% and 58.3%, respectively. On the contrary, FADOH combined with ITC showed poor synergistic inhibitory activity (16.7%) against . Moreover, the additive rates of these two drugs against , , and were 25%, 41.7%, and 33.3%, respectively. No antagonistic interactions were observed. The drop-plate assay and time-growth curves confirmed that the combination of FADOH and ITC had a potent synergistic antifungal effect. The synergistic effect of FADOH and ITC against dermatophytes is reported here for the first time. Our findings suggest the potential use of FADOH as an effective antifungal drug in the combined therapy of dermatophytoses caused especially by and .
先前的研究表明，天然多炔醇类物质，如法落醇（FADOH），对植物真菌具有良好的抗真菌作用。然而，其对感染人类的真菌的作用仍有待探索。在我们的研究中，棋盘微量稀释法、滴板法和时间生长法被用来分析 FADOH 与伊曲康唑（ITC）对皮肤真菌（包括 12 株[Trichophyton rubrum]、12 株[Trichophyton mentagrophytes]和 6 株[Trichophyton tonsurans]）的相互作用。结果表明，FADOH 与 ITC 联合对所有测试的皮肤真菌的 86.7%表现出协同和相加作用。FADOH 对 ITC 对 和 的协同作用极好；协同率分别为 66.7%和 58.3%。相反，FADOH 与 ITC 联合对 表现出较差的协同抑制活性（16.7%）。此外，这两种药物对 、 、和 的相加率分别为 25%、41.7%和 33.3%。未观察到拮抗相互作用。滴板法和时间生长曲线证实了 FADOH 和 ITC 联合具有强大的协同抗真菌作用。这是首次报道 FADOH 和 ITC 对皮肤真菌具有协同作用。我们的研究结果表明，FADOH 作为一种有效的抗真菌药物，在由 和 引起的皮肤真菌病的联合治疗中具有潜在的应用价值。
