Department of Chemistry, Renmin University of China, Beijing 100872, China.
CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
ACS Macro Lett. 2023 Jun 20;12(6):745-750. doi: 10.1021/acsmacrolett.3c00183. Epub 2023 May 19.
Nebulized lipid nanoparticles (LNPs) have been considered as potential therapies for genetic disease as well as infectious disease. However, the sensitivity of LNPs to high shear stress during the nebulization process results in loss of the integrity of the nanostructure and the capability of delivering active pharmaceutical ingredients. Herein we have provided a fast extrusion method to prepare liposomes incorporated with a DNA hydrogel (hydrogel-LNPs) to improve the stability of the LNPs. Taking advantage of the good cellular uptake efficiency, we also demonstrated the potential of hydrogel-LNPs in delivering small molecular doxorubicin (Dox) and nucleic acid drugs. This work provides not only highly biocompatible hydrogel-LNPs for aerosol delivery, but also a strategy to regulate the elasticity of LNPs, which will benefit the potential optimization of drug delivery carriers.
雾化脂质纳米颗粒（LNPs）已被认为是治疗遗传疾病和传染病的潜在疗法。然而，LNPs 在雾化过程中对高剪切应力非常敏感，导致纳米结构的完整性和活性药物成分的传递能力丧失。在此，我们提供了一种快速挤出的方法来制备包载 DNA 水凝胶的脂质体（水凝胶-LNPs），以提高 LNPs 的稳定性。利用良好的细胞摄取效率，我们还证明了水凝胶-LNPs 递送小分子阿霉素（Dox）和核酸药物的潜力。这项工作不仅为气溶胶递药提供了高度生物相容的水凝胶-LNPs，还提供了一种调节 LNPs 弹性的策略，这将有利于药物传递载体的潜在优化。
