Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India; Nanobiotechnology Laboratory, Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Int J Biol Macromol. 2023 Jul 1;242(Pt 3):124827. doi: 10.1016/j.ijbiomac.2023.124827. Epub 2023 May 17.
The development of 3D scaffold-based in vitro tumor models can help to address the limitations of cell culture and animal models for designing and screening of anticancer drugs. In this study, in vitro 3D tumor models using sodium alginate (SA) and sodium alginate/silk fibroin (SA/SF) porous beads were developed. The beads were non-toxic and A549 cells had a high tendency to adhere, proliferate, and form tumor-like aggregates within SA/SF beads. The 3D tumor model based on these beads had better efficacy for anti-cancer drug screening than the 2D cell culture model. Additionally, the SA/SF porous beads loaded with superparamagnetic iron oxide nanoparticles were used to explore their magneto-apoptosis ability. The cells exposed to a high magnetic field were more likely to undergo apoptosis than those exposed to a low magnetic field. These findings suggest that the SA/SF porous beads and SPIONs loaded SA/SF porous beads-based tumor models could be useful for drug screening, tissue engineering, and mechanobiology studies.
基于 3D 支架的体外肿瘤模型的发展有助于解决细胞培养和动物模型在设计和筛选抗癌药物方面的局限性。在这项研究中，使用海藻酸钠 (SA) 和海藻酸钠/丝素蛋白 (SA/SF) 多孔珠开发了体外 3D 肿瘤模型。这些珠子是无毒的，A549 细胞有很高的倾向在 SA/SF 珠内附着、增殖并形成肿瘤样聚集物。基于这些珠子的 3D 肿瘤模型在抗癌药物筛选方面比 2D 细胞培养模型更有效。此外，还使用负载超顺磁性氧化铁纳米粒子的 SA/SF 多孔珠来探索它们的磁凋亡能力。暴露于强磁场的细胞比暴露于低磁场的细胞更容易发生凋亡。这些发现表明，SA/SF 多孔珠和负载 SA/SF 多孔珠的 SPIONs 肿瘤模型可用于药物筛选、组织工程和机械生物学研究。
