State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, People's Republic of China.
International School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2022 Dec;110(12):2667-2675. doi: 10.1002/jbm.b.35119. Epub 2022 Jun 27.
Engineering skin substitutes represent a prospective source of advanced therapy in repairing severe traumatic wounds. Sodium alginate (SA) and silk fibroin (SF) are natural biomaterials, which are widely used in tissue engineering and other fields because of their low price, high safety, and good biocompatibility. However, SA itself degrades slowly, its degradation mode is difficult to control, and the degradation products are difficult to remove from the body because of its high molecular weight. Therefore, the composite scaffolds were prepared by freeze-drying composite technology by using the Schiff base reaction between biocompatible SF and permeable oxidized sodium alginate (OSA). Sodium periodate was used as oxidant to modify SA. The results showed that higher oxidation degree of OSA could be obtained by increasing the proportion of oxidant, and the relative molecular weight of the oxidized products could also be reduced. The composite scaffolds were prepared by using sodium tetraborate as a crosslinking accelerator of the Schiff base reaction between OSA and SF. FT-IR confirmed that the Schiff base group appeared in the material. In vitro biodegradation experiments showed that the biodegradation of the composite scaffolds was controllable, and the cytocompatibility experiment showed that the composite scaffolds had good biocompatibility.
工程化皮肤替代物是修复严重创伤的先进治疗的潜在来源。海藻酸钠（SA）和丝素蛋白（SF）是天然生物材料，由于其价格低廉、安全性高、生物相容性好，广泛应用于组织工程等领域。然而，SA 本身降解缓慢，其降解模式难以控制，并且由于其分子量高，其降解产物难以从体内去除。因此，采用生物相容性 SF 与可渗透氧化海藻酸钠（OSA）之间的席夫碱反应，通过冷冻干燥复合技术制备了复合支架。用过碘酸钠作为氧化剂对 SA 进行修饰。结果表明，通过增加氧化剂的比例可以获得更高的 OSA 氧化程度，并且氧化产物的相对分子量也可以降低。采用四硼酸钠作为 OSA 和 SF 之间席夫碱反应的交联促进剂制备了复合支架。FT-IR 证实了材料中出现了席夫碱基团。体外生物降解实验表明，复合支架的生物降解是可控的，细胞相容性实验表明，复合支架具有良好的生物相容性。
