Elektronic-Automation Department, Vocational School of Health Services, Harran University, 63300, Sanlıurfa, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Adıyaman University, 02000, Adıyaman, Turkey.
Eur Spine J. 2023 Jul;32(7):2368-2376. doi: 10.1007/s00586-023-07757-7. Epub 2023 May 19.
Oxidative stress in the vertebral endplates of patients with low back pain and Modic changes (MCs) (types I, II, and III) endplate changes on magnetic resonance imaging. 8-iso-prostaglandin F (8-iso-PGF) has been proposed as new indicator of oxidative stress. Raftlin, as an inflammatory biomarker, has been previously reported in inflammatory diseases. Oxidative stress plays an important role in various human diseases. This study was aimed to assess Raftlin and 8-iso-PGF levels in patients with MCs.
Patients with MCI, II, and III (n = 45) and age- and sex matched controls subjects (n = 45) were enrolled in this study. 8-iso-PGF and Raftlin levels in the serum samples of both groups were measured with enzyme-linked immunosorbent assay.
In our study results, raftlin levels changed in parallel with prostaglandin levels (p < 0.05). Raftlin levels changed in parallel with prostaglandin levels (p < 0.05). The levels of 8-iso-PGF and Raftlin levels showed increase in patients with MCs and the control group (p < 0.05). In addition, a significant positive correlation was found between MC-I, MC-II, MC-III and Raftlin (r = 0.756, 0.733, 0.701 p < 0.001, respectively). A significant positive correlation was found between ISO (Respectively; r = 0.782, 0.712, 0.716 p < 0.001). In our evaluation between Raftlin and Iso, a significant positive relationship was determined. (r = 0.731, p < 0.001).
Our findings indicated that oxidative stress in patients with MC-I may be aggravated and it may cause an inflammation formation of the lesion areas in these patients. Also, the increased 8-iso-PGF and Raftlin levels in patients with MC-II and MC-III may be an adaptive response to against oxidative stress.
研究氧化应激在腰痛伴 Modic 改变（MCs）（I、II 和 III 型）患者的椎骨终板和磁共振成像上终板改变中的作用。8-异前列腺素 F（8-iso-PGF）已被提出作为氧化应激的新指标。Raftlin 作为一种炎症生物标志物，先前已在炎症性疾病中报道过。氧化应激在各种人类疾病中起着重要作用。本研究旨在评估 MCs 患者的 Raftlin 和 8-iso-PGF 水平。
本研究纳入了 45 例 MCs I、II 和 III 患者（n=45）和年龄及性别匹配的对照组受试者（n=45）。使用酶联免疫吸附试验测量两组血清样本中的 8-iso-PGF 和 Raftlin 水平。
在我们的研究结果中，Raftlin 水平与前列腺素水平平行变化（p<0.05）。Raftlin 水平与前列腺素水平平行变化（p<0.05）。MCs 患者和对照组的 8-iso-PGF 和 Raftlin 水平均升高（p<0.05）。此外，MC-I、MC-II 和 MC-III 与 Raftlin 之间存在显著正相关（r=0.756、0.733、0.701，p<0.001）。ISO 与 Raftlin 之间存在显著正相关（分别为 r=0.782、0.712、0.716，p<0.001）。在我们对 Raftlin 和 Iso 之间的评估中，确定了显著的正相关关系。（r=0.731，p<0.001）。
我们的研究结果表明，MC-I 患者的氧化应激可能加重，这可能导致这些患者病变区域的炎症形成。此外，MC-II 和 MC-III 患者中 8-iso-PGF 和 Raftlin 水平的增加可能是对氧化应激的适应性反应。
