Ash C, Atkinson H J
Exp Parasitol. 1986 Aug;62(1):24-8. doi: 10.1016/0014-4894(86)90004-4.
Thermistors and an amplified bridge were used to detect supercooling points of Nematodirus battus eggs weighing ca. 1 microgram wet weight. A cooling rate of about 1 C min-1 was achieved with a manually controlled cold stage using the Peltier effect. The supercooling point of eggs fell during chilling at 5 +/- 1 C for up to 8 weeks from -34.48 +/- 0.49 C to -37.17 +/- 0.76 C. Juveniles freed from these eggs were less cold hardy than intact eggs but chilling improved their supercooling to a greater extent from -19.33 +/- 1.38 C to -32.10 +/- 0.68 C. These results were obtained with eggs showing the characteristic hatching response for this species after transfer from chilling at 5 C to higher temperatures (5-37 +/- 1 C). The results indicate eggs of N. battus acclimate to chilling at a time when previous work had established an increase in their trehalose content.
热敏电阻和一个放大电桥被用于检测重约1微克湿重的巴氏细颈线虫卵的过冷点。利用珀尔帖效应，通过手动控制的冷台实现了约1℃/分钟的冷却速率。在5±1℃下冷藏长达8周期间，虫卵的过冷点从-34.48±0.49℃降至-37.17±0.76℃。从这些虫卵中孵出的幼虫比完整虫卵的耐寒性差，但冷藏使它们的过冷点有了更大程度的改善，从-19.33±1.38℃提高到-32.10±0.68℃。这些结果是在用从5℃冷藏转移到较高温度（5 - 37±1℃）后表现出该物种典型孵化反应的虫卵获得的。结果表明，巴氏细颈线虫的虫卵在之前的研究已确定其海藻糖含量增加的时期适应了冷藏。
