Girich Alexander, Sadriev Konstantin, Frolova Lidia, Dolmatov Igor
A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
Institute of the World Ocean, Far Eastern Federal University (FEFU), Vladivostok, Russia.
Wound Repair Regen. 2023 Jul-Aug;31(4):464-474. doi: 10.1111/wrr.13097. Epub 2023 May 26.
The secreted frizzled-related proteins (sfrp) and smoothened (smo) genes and their possible role in the regeneration of internal organs in the holothurian Eupentacta fraudatrix were studied. In this species, two sfrp genes were identified: sfrp1/2/5, sfrp3/4 and one smo gene. Their expression was analysed during regeneration of the aquapharyngeal bulb (AB) and intestine, and these genes were knock down by RNA interference. It has been shown that the expression of these genes is extremely important for the formation of AB. In all animals subjected to knockdown, at 7 days after evisceration, a full-sized AB rudiment was not formed. As a result of sfrp1/2/5 knockdown, the process of extracellular matrix remodelling in AB is interrupted, that leading to clusters of dense connective tissue formation, which slows down cell migration. When sfrp3/4 is knockdown, the connective tissue of AB anlage is completely disrupted and its symmetry is broken. The effect of smo knockdown was expressed in a significant impairment of AB regeneration, when connections between ambulacras were not formed after evisceration. However, despite severe disturbances in AB regeneration, a normal-sized gut anlage developed in all cases, which suggests that the regeneration of the digestive tube and AB occur independently of each other.
研究了分泌型卷曲相关蛋白（sfrp）和平滑蛋白（smo）基因及其在刺参（Eupentacta fraudatrix）内脏器官再生中的可能作用。在该物种中，鉴定出两个sfrp基因：sfrp1/2/5、sfrp3/4和一个smo基因。分析了它们在水管咽球（AB）和肠道再生过程中的表达，并通过RNA干扰敲低了这些基因。结果表明，这些基因的表达对AB的形成极为重要。在所有接受敲低处理的动物中，去脏后7天未形成完整大小的AB原基。sfrp1/2/5敲低的结果是，AB中细胞外基质重塑过程被中断，导致致密结缔组织簇形成，从而减缓细胞迁移。当sfrp3/4被敲低时，AB原基的结缔组织完全被破坏，其对称性被打破。smo敲低的影响表现为AB再生明显受损，去脏后步带之间未形成连接。然而，尽管AB再生受到严重干扰，但在所有情况下都发育出了正常大小的肠原基，这表明消化管和AB的再生是相互独立发生的。
