College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, China.
College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, China; School of Environment Science and Engineering, Shandong University, Qingdao, Shandong Province 266237, China.
Sci Total Environ. 2023 Sep 1;889:164290. doi: 10.1016/j.scitotenv.2023.164290. Epub 2023 May 19.
Nitrification is an important link for environmental nitrogen cycling, the appearance of comammox updates our traditional cognition about nitrification. Yet comammox has been poorly studied in marine sediments. This study explored the differences in abundance, diversity, community structure of comammox cladeA amoA in the sediments from different offshore areas of China (the Bohai Sea (BS), the Yellow Sea (YS), and the East China Sea (ECS)) and revealed the main driving factors. The abundance of comammox cladeA amoA was 8.11 × 10-4.96 × 10, 2.85 × 10-4.18 × 10, and 5.76 × 10-4.91 × 10 copies/g dry sediment in BS, YS, and ECS, respectively. The OTU (operational taxonomic units) numbers of comammox cladeA amoA in the BS, YS, and ECS were 4, 2, and 5 respectively. There were negligible differences in the abundance and diversity of comammox cladeA amoA among the sediments of the three seas. The subclade of comammox cladeA amoA, cladeA2 is the dominant comammox flora in the offshore area sediments of China. Noticeable differences in the community structure of comammox were observed among these three seas, where the relative abundance of cladeA2 in comammox was 62.98 %, 66.24 %, and 100 % in ECS, BS, and YS respectively. pH was found as the main factor affecting the abundance of comammox cladeA amoA and showed a significant positive correlation (p < 0.05). The diversity of comammox decreased with the increase of salinity (p < 0.05). NO-N is the main factor affecting the community structure of comammox cladeA amoA.
硝化作用是环境氮循环的重要环节， comammox 的出现更新了我们对硝化作用的传统认知。然而， comammox 在海洋沉积物中的研究还很不完善。本研究探讨了中国不同近海海域（渤海（BS）、黄海（YS）和东海（ECS））沉积物中 comammox 科 A amoA 的丰度、多样性和群落结构的差异，并揭示了主要的驱动因素。BS、YS 和 ECS 沉积物中 comammox 科 AamoA 的丰度分别为 8.11×10-4.96×10、2.85×10-4.18×10 和 5.76×10-4.91×10 拷贝/g 干沉积物。BS、YS 和 ECS 沉积物中 comammox 科 AamoA 的 OTU（操作分类单元）数量分别为 4、2 和 5。这三个海域沉积物中 comammox 科 AamoA 的丰度和多样性差异可忽略不计。comammox 科 AamoA 的亚科，A2 是中国近海沉积物中主要的 comammox 菌群。这三个海域的 comammox 群落结构存在明显差异，其中 ECS、BS 和 YS 中 comammox 的相对丰度分别为 62.98%、66.24%和 100%。pH 被发现是影响 comammox 科 AamoA 丰度的主要因素，呈显著正相关（p<0.05）。comammox 的多样性随着盐度的增加而降低（p<0.05）。NO-N 是影响 comammox 科 AamoA 群落结构的主要因素。
